A New Aminopeptidase from the Keratin-Degrading Strain Streptomyces fradiae var. k11

文献类型： 外文期刊

第一作者： Wu, Bo

作者： Wu, Bo;Shi, Pengjun;Wang, Yaru;Meng, Kun;Bai, Yingguo;Luo, Huiying;Yang, Peilong;Zhou, Zhigang;Yao, Bin;Li, Jiang

作者机构：

关键词： Aminopeptidase;Streptomyces fradiae var. k11;C-terminal propeptide

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN： 0273-2289

年卷期： 2010 年 160 卷 3 期

页码：

收录情况： SCI

摘要： An aminopeptidase gene fragment was isolated from a keratin-degrading strain, Streptomyces fradiae var. k11, by PCR amplification using a degenerate primer set designed based on the partial amino acid sequence of the native enzyme. The gene, designated sfap, encoded a polypeptide of 461 amino acids comprised of three domains: a signal peptide, a mature region, and a C-terminal propeptide. The aminopeptidase, SFAP, had highest amino acid sequence identity (79%) with a putative aminopeptidase from Streptomyces griseus subsp. griseus NBRC 13350. The gene with and without C-terminal propeptide was successfully overexpressed in Escherichia coli BL21 (DE3), and the gene without C-terminal propeptide encoded a functional enzyme. Purified recombinant SFAP exhibited optimal activity at pH 8.0 and 60 A degrees C, and retained > 60% peak activity over a broad range of temperature. The enzyme was thermal and pH stable, and showed metalloprotease characteristics, which was inhibited by EDTA but activated by Ca2+ and Co2+. This is the first study to report the gene cloning and expression of a leucine aminopeptidase from S. fradiae.

分类号：

  • 相关文献

[1]Effects of a protease inhibitor protein from Xenorhabdus bovienii on physiology of pea aphid (Acyrthosiphon pisum). Zeng, Fanrong,Zhang, Heqing,Jin, Danjuan,Dong, Shuanglin. 2014

[2]Identification of an aminopeptidase from the skeletal muscle of grass carp (Ctenopharyngodon idellus). Liu, Bing-Xin,Sun, Le-Chang,Su, Wen-Jin,Cao, Min-Jie,Zhou, Li-Gen,Hara, Kenji.

作者其他论文 更多>>

微信小程序