Development and evaluation of a VP3-ELISA for the detection of goose and Muscovy duck parvovirus antibodies

文献类型: 外文期刊

第一作者: Zhang, Yun

作者: Zhang, Yun;Liu, Ming;Zhang, Dabing;Li, Gang;Tong, Guangzhi

作者机构:

关键词: Goose parvovirus;Muscovy duck parvovirus;VP3 protein;ELISA;Cross-reactivity

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN: 0166-0934

年卷期: 2010 年 163 卷 2 期

页码:

收录情况: SCI

摘要: The VP3-encoding gene of goose parvovirus (GPV) Ep22 strain was cloned and expressed in Escherichia coli. The GPV VP3-encoding gene was 1605 bp in length, and it encoded a 534 amino acid protein with a predicted molecular mass of 59.9kDa. The VP3 fusion protein expressed in E. coli was detected by goose and Muscovy duck anti-parvovirus polyclonal sera. in addition, an ELISA (VP3-ELISA) using the VP3 protein as the coating antigen for the detection of antibodies to GPV in geese and antibodies to Muscovy duck parvovirus (MDPV) in Muscovy ducks was developed. Compared to the virus neutralization test, the specificity and sensitivity of the VP3-ELISA was 90.2% and 95.2% for goose sera and 91.8% and 96.7% for Muscovy duck sera, respectively. The VP3-ELISA did not react with the anti-sera to other goose or duck pathogens, indicating that this protein is specific for the reorganization of goose or duck anti-parvovirus antibodies. Cross-reactivity between immunoglobulin G antibodies from geese and Muscovy ducks was also tested, and the results reflected the phylogenetic distance between these two birds when using the ELISA. In conclusion, the VP3-ELISA is a sensitive and specific method for detecting antibodies against GPV or MDPV. (C) 2009 Elsevier B.V. All rights reserved.

分类号:

  • 相关文献

[1]Identification of a recombinant Muscovy Duck parvovirus (MDPV) in Shanghai, China. Zhu, Yumin,Zhou, Zongqing,Huang, Yu,Yu, Ruisong,Dong, Shijuan,Li, Zhen,Zhu, Yumin,Zhou, Zongqing,Huang, Yu,Yu, Ruisong,Dong, Shijuan,Li, Zhen,Zhang, Yuanshu.

[2]Genomic Characterization of Goose Parvovirus and Muscovy Duck Parvovirus Co-infection in Fujian, China. Wan, Chun-he,Hu, Kai-hui,Wan, Chun-he,Chen, Hong-mei,Fu, Qiu-ling,Fu, Guang-hua,Cheng, Long-fei,Shi, Shao-hua,Huang, Yu. 2015

[3]Phylogenetic analysis of VP1 gene sequences of waterfowl parvoviruses from the Mainland of China revealed genetic diversity and recombination. Wang, Shao,Cheng, Xiao-xia,Chen, Shao-ying,Lin, Feng-qiang,Chen, Shi-long,Zhu, Xiao-li,Wang, Jin-xiang,Huang, Mei-qing,Zheng, Min,Wang, Shao,Cheng, Xiao-xia,Chen, Shao-ying,Lin, Feng-qiang,Chen, Shi-long,Zhu, Xiao-li,Wang, Jin-xiang,Huang, Mei-qing,Zheng, Min.

[4]Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses. Wan, Chun-He,Chen, Hong-Mei,Fu, Qiu-Ling,Shi, Shao-Hua,Fu, Guang-Hua,Cheng, Long-Fei,Chen, Cui-Teng,Huang, Yu,Hu, Kai-Hui.

[5]Establishment of a sandwich enzyme-linked immunosorbent assay for specific detection of Bacillus thuringiensis (Bt) Cry1Ab toxin utilizing a monoclonal antibody produced with a novel hapten designed with molecular model. Dong, Sa,Zhang, Xiao,Liu, Yuan,Zhang, Cunzheng,Xie, Yajing,Zhong, Jianfeng,Xu, Chongxin,Liu, Xianjin,Dong, Sa,Liu, Xianjin.

[6]Serological detection of bovine ephemeral fever virus using an indirect ELISA based on antigenic site G(1) expressed in Pichia pastoris. Qiu, Chang-Qing.

[7]Production of ultrasensitive generic monoclonal antibodies against major aflatoxins using a modified two-step screening procedure. Li, Peiwu,Li, Peiwu,Zhang, Qi,Zhang, Wen,Huang, Yanling,Ding, Xiaoxia,Jiang, Jun.

[8]A SERS-based multiple immuno-nanoprobe for ultrasensitive detection of neomycin and quinolone antibiotics via a lateral flow assay. Shi, Qiaoqiao,Zhang, Gaiping,Shi, Qiaoqiao,Sun, Yaning,Deng, Ruiguang,Teng, Man,Li, Qingmei,Yang, Yanyan,Hu, Xiaofei,Zhang, Gaiping,Shi, Qiaoqiao,Huang, Jie,Zhang, Zhijun,Zhang, Gaiping. 2018

[9]Selection of an aptamer against Muscovy duck parvovirus for highly sensitive rapid visual detection by label-free aptasensor. Lu, Taofeng,Ma, Qin,Yan, Wenzhuo,Wang, Yuanzhi,Zhang, Yuanyuan,Zhao, Lili,Chen, Hongyan. 2018

[10]Genomic and pathogenic analysis of a Muscovy duck parvovirus strain causing short beak and dwarfism syndrome without tongue protrusion. Fu, Qiuling,Huang, Yu,Wan, Chunhe,Fu, Guanghua,Cheng, Longfei,Shi, Shaohua,Chen, Hongmei,Liu, Rongchang,Chen, Zhenhai,Qin, Baomin.

[11]Novel duck parvovirus identified in Cherry Valley ducks (Anas platyrhynchos domesticus), China. Li, Chuanfeng,Li, Qi,Chen, Zongyan,Liu, Guangqing.

[12]Genetic Characterization of a Potentially Novel Goose Parvovirus Circulating in Muscovy Duck Flocks in Fujian Province, China. Wang, Shao,Cheng, Xiao-Xia,Chen, Shao-Ying,Zhu, Xiao-Li,Chen, Shi-Long,Lin, Feng-Qiang,Li, Zhao-Long,Wang, Shao,Cheng, Xiao-Xia,Chen, Shao-Ying,Chen, Shi-Long,Lin, Feng-Qiang. 2013

[13]Rapid diagnosis of goose viral infections by multiplex PCR. Chen, Zongyan,Li, Chuanfeng,Li, Guoxin,Yu, Hai,Jiang, Yifeng,Yan, Liping,Meng, Chunchun,Zhou, Yanjun,Tong, Guangzhi,Liu, Guangqing.

[14]Complete Genome Sequence of Goose Parvovirus Isolated from Anser cygnoides in China. Wan, Chun-he,Hu, Kai-hui,Wan, Chun-he,Huang, Yu,Shi, Shao-hua,Fu, Guang-hua,Cheng, Long-fei,Chen, Hong-mei,Fu, Qiu-ling,Chen, Cui-teng. 2016

[15]Evidence for natural recombination in the capsid gene VP2 of Taiwanese goose parvovirus. Wang, Shao,Cheng, Xiaoxia,Chen, Shaoying,Lin, Fengqiang,Chen, Shilong,Zhu, Xiaoli,Wang, Jingxiang,Wang, Shao,Cheng, Xiaoxia,Chen, Shaoying,Lin, Fengqiang,Chen, Shilong,Zhu, Xiaoli,Wang, Jingxiang.

[16]Expression, Purification, Characterization and Subcellular Localization of the Goose Parvovirus Rep1 Protein. Chen, Zongyan,Li, Chuanfeng,Peng, Gaojing,Liu, Guangqing. 2013

[17]Comparative genetic analysis and pathological characteristics of goose parvovirus isolated in Heilongjiang, China. Niu, Yinjie,Zhao, Lili,Liu, Baihan,Yin, Haichang,Huo, Hong,Chen, Hongyan,Liu, Baihan,Liu, Jingli,Yang, Fan. 2018

[18]Immunogenicity of virus-like particles containing modified goose parvovirus VP2 protein. Chen, Zongyan,Li, Chuanfeng,Zhu, Yingqi,Wang, Binbin,Meng, Chunchun,Liu, Guangqing.

[19]Cloning of the genome of a goose parvovirus vaccine strain SYG61v and rescue of infectious virions from recombinant plasmid in embryonated goose eggs. Wang, Jianye,Duan, Jinkun,Meng, Xia,Jiang, Zhiwei,Zhu, Guoqiang,Wang, Jianye,Duan, Jinkun,Meng, Xia,Jiang, Zhiwei,Zhu, Guoqiang,Gong, Jiansen. 2014

[20]Generation and evaluation of a recombinant genotype VII Newcastle disease virus expressing VP3 protein of Goose parvovirus as a bivalent vaccine in goslings. Wang, Jianzhong,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Wang, Jianzhong,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Wang, Chunfeng,Feng, Na,Yang, Songtao,Xia, Xianzhu,Xiao, Yueqiang,Wang, Wenxiu,Liu, Xiufan,Hu, Shunlin,Ding, Chan,Yu, Shengqing.

作者其他论文 更多>>