Development and evaluation of a VP3-ELISA for the detection of goose and Muscovy duck parvovirus antibodies
文献类型: 外文期刊
第一作者: Zhang, Yun
作者: Zhang, Yun;Liu, Ming;Zhang, Dabing;Li, Gang;Tong, Guangzhi
作者机构:
关键词: Goose parvovirus;Muscovy duck parvovirus;VP3 protein;ELISA;Cross-reactivity
期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )
ISSN: 0166-0934
年卷期: 2010 年 163 卷 2 期
页码:
收录情况: SCI
摘要: The VP3-encoding gene of goose parvovirus (GPV) Ep22 strain was cloned and expressed in Escherichia coli. The GPV VP3-encoding gene was 1605 bp in length, and it encoded a 534 amino acid protein with a predicted molecular mass of 59.9kDa. The VP3 fusion protein expressed in E. coli was detected by goose and Muscovy duck anti-parvovirus polyclonal sera. in addition, an ELISA (VP3-ELISA) using the VP3 protein as the coating antigen for the detection of antibodies to GPV in geese and antibodies to Muscovy duck parvovirus (MDPV) in Muscovy ducks was developed. Compared to the virus neutralization test, the specificity and sensitivity of the VP3-ELISA was 90.2% and 95.2% for goose sera and 91.8% and 96.7% for Muscovy duck sera, respectively. The VP3-ELISA did not react with the anti-sera to other goose or duck pathogens, indicating that this protein is specific for the reorganization of goose or duck anti-parvovirus antibodies. Cross-reactivity between immunoglobulin G antibodies from geese and Muscovy ducks was also tested, and the results reflected the phylogenetic distance between these two birds when using the ELISA. In conclusion, the VP3-ELISA is a sensitive and specific method for detecting antibodies against GPV or MDPV. (C) 2009 Elsevier B.V. All rights reserved.
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