Proteomics reveals the effects of gibberellic acid (GA(3)) on salt-stressed rice (Oryza sativa L.) shoots

文献类型: 外文期刊

第一作者: Wen, Fu-ping

作者: Wen, Fu-ping;Zhang, Zhao-hui;Bai, Ting;Xu, Qin;Pan, Ying-hong

作者机构:

关键词: Gibberellic acid (GA3);Salt stress;Proteomics;Rice (Oryza sativa L.)

期刊名称:PLANT SCIENCE ( 影响因子:4.729; 五年影响因子:5.132 )

ISSN: 0168-9452

年卷期: 2010 年 178 卷 2 期

页码:

收录情况: SCI

摘要: Salt decreases gibberellic acid (GA(3)) content in rice. Nevertheless, GA(3) may exert a beneficial effect on salt-stressed rice. in our analysis, GA(3) reduced NaCl-induced growth inhibition in rice (Oryza sativa L. cv. Nipponbare) in a concentration-dependent manner, including the length of root tissue. However, remains unclear. We employed a comparative proteomic analysis to elucidate the mechanism of GA(3) activity in this phenomenon. 5-Day-old seedlings treated with salt and gibberellic acid (H2O, 0.5% NaCl, 0.5% NaCl + 100 mu M GA(3), and 100 mu M GA(3)) for 48 h were used for the proteomics analysis. Eleven proteins differently regulated by salt and GA(3) were revealed by 2D PAGE and were identified by MALDI-TOF MS. These proteins were identified as glutamyl-tRNA reductase, enolase, salt stress-induced protein (SALT protein), Os09g0249700, hypothetical protein OsJ_014066, putative chaperonin 21 precursor, Os04g0659300, hypothetical protein OsJ_025258, ribulose bisphosphate carboxylase, isoflavone reductase-like protein and phosphoglucomutase. Some of these proteins are involved in biochemical pathways such as photosynthesis and glycolysis, but others were found to be novel proteins involved specifically in the response to salt in rice. GA(3) had a significant influence on the abundance of some salt-regulated proteins. Our studies have provided new insight to reveal the modulating effect of GA(3) on salt stress in rice. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

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