文献类型： 外文期刊

第一作者： Jing Zhi-zhong

作者： Jing Zhi-zhong;Dou Yong-xi;Meng Xue-lian;Chen Guo-hua;Wang Pei-ya;Luo Qi-hui;Yuan Gai-ling;Zheng Ya-dong;Cai Xue-peng

作者机构：

关键词： porcine cytokines;Cysticercosis cellulosae;adjuvant effect;immunity response

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 1 期

页码：

收录情况： SCI

摘要： To investigate the adjuvant potential of porcine IL-4 and IFN-gamma, in mice and pigs, the genes of porcine IL-4 and IFN-gamma were cloned and the recombinant mammalian expression plasmids were constructed for in vivo expression of the cytokines. Adjuvant effects of recombinant expression plasmids of IL-4 and IFN-gamma (pcDNA-IL-4, pcDNA-IFN-gamma) co-administrated with Cysticercus cellulosae crude antigen or TSOLI8 recombinant protein antigen have been carried out in mice and pigs, respectively. We have demonstrated that recombinant plasmids of the cytokines as an adjuvant could induce stronger immune response in mice and pigs. With the C. cellulosae parasite antigen, porcine pcDNA-IL-4 induced higher specific antibody of immunized mice than pcDNA-IFN-gamma. But pcDNA-IFN-y is significantly stronger than that of no adjuvant or empty plasmids with the antigen control group. For the TSOLI8 recombinant protein antigen vaccine, pcDNA-IL-4 still had a stronger ability to enhance specific antibody in swine than pcDNA-IFN-y (P < 0.01), but the immune protective rate was lower in challenged pigs (only 68.7%). Although pcDNA-IFN-gamma showed lower specific antibody, the protection rate was very high (91%) than other group (P < 0.01). This study indicated that the recombinant expression plasmids of porcine IL-4 and IFN-gamma display stronger adjuvant effects to C. cellulosae vaccine, further research should be carried out for understanding of the interaction mechanism.

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