Establishment and Characterization of Fibroblast Cell Line Derived from Siberian Tiger (Panthera tigris altaica)

文献类型： 外文期刊

第一作者： Liu, Changqing

作者： Liu, Changqing;Guan, Weijun;Ma, Yuehui;Liu, Changqing;Guo, Yu;Liu, Dan

作者机构：

期刊名称：BIOPRESERVATION AND BIOBANKING （ 影响因子：2.3； 五年影响因子：2.418 ）

ISSN： 1947-5543

年卷期： 2010 年 8 卷 2 期

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收录情况： SCI

摘要： The Siberian tiger ear marginal tissue fibroblast cell line (STF34) from 34 samples was successfully established using primary explants technique and cell cryoconservation technology. STF34 cells were adherent, with a population doubling time of 24 h. Chromosome analysis showed that 90.2%-91.6% of cells were diploid (2n = 38). Isoenzyme analyses of lactate dehydrogenase and malate dehydrogenase showed that STF34 cells had no cross-contamination with other species. Tests for cell line contamination with bacteria, fungi, viruses, and mycoplasmas were all negative. Every index of the STF34 cell line meets all the standard quality controls of American Type Culture Collection. Not only has the germline of this important Siberian tiger species been preserved at the cell level, but also valuable material had been provided for genome, postgenome, and soma-cloning research.

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