Influence of cellulase gene knockdown by dsRNA interference on the development and reproduction of the pine wood nematode, Bursaphelenchus xylophilus
文献类型: 外文期刊
第一作者: Dai, Su-Ming
作者: Dai, Su-Ming;Xiao, Luo;Xie, Bing-Yan;Cheng, Xin-Yue
作者机构:
关键词: beta-1,4-endoglucanases gene;gene silencing;plant-parasitic nematodes;RNA interference
期刊名称:NEMATOLOGY ( 影响因子:1.442; 五年影响因子:1.485 )
ISSN: 1388-5545
年卷期: 2010 年 12 卷
页码:
收录情况: SCI
摘要: Cellulase genes are very important for plant-parasitic nematodes to move and feed within their host plants. The pine wood nematode, Bursaphelenchus xylophilus, causes destructive damage by killing pine trees. In this study, by employing dsRNA interference technology, knockdown of a cellulase gene (Bx-eng-1) of B. xylophilus was achieved and the biological effects of RNAi on the nematode were observed. The result showed that, after 24 h soaking, dsRNA of the Bx-eng-1 gene was effectively delivered into the nematode causing a post-transcriptional gene silencing and decrease in cellulase activity. Moreover, the number of F1 generation offspring was reduced significantly when the dsRNA-treated nematodes were cultured on fungal mats. We consider that cellulase is important to B. xylophilus because it not only hydrolyses cellulose of plant cell wall for its parasitism and penetration in host plants, but also influences its feeding, development and propagation on fungal mats.
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