Immunization by influenza virus-like particles protects aged mice against lethal influenza virus challenge
文献类型: 外文期刊
第一作者: Wen, Zhiyuan
作者: Wen, Zhiyuan;Ye, Ling;Gao, Yulong;Pan, Lei;Dong, Ke;Compans, Richard W.;Yang, Chinglai;Wen, Zhiyuan;Ye, Ling;Gao, Yulong;Pan, Lei;Dong, Ke;Compans, Richard W.;Yang, Chinglai;Wen, Zhiyuan;Gao, Yulong;Bu, Zhigao;Pan, Lei;Dong, Ke
作者机构:
关键词: Influenza;Virus-like particle;Aging;Antibody response;Avidity
期刊名称:ANTIVIRAL RESEARCH ( 影响因子:5.97; 五年影响因子:5.801 )
ISSN: 0166-3542
年卷期: 2009 年 84 卷 3 期
页码:
收录情况: SCI
摘要: Influenza virus-like particles (VLPs) were produced in Sf9 insect cells by co-expressing the matrix protein M1 and the surface glycoproteins hemagglutinin (HA) and neuraminidase (NA) using the recombinant baculovirus expression system. The VLPs were morphologically similar to influenza virions. Both HA and NA proteins were incorporated into VLPs and these proteins retained their functional activities. Further, influenza VLPs but not inactivated influenza viruses (IIV) stimulated secretion of inflammatory cytokines from mouse bone marrow-derived dendritic cells (BMDC). Immunogenicity of influenza VLPs and their protective efficacies against lethal influenza virus challenge were evaluated in young and aged mice. Immunization with influenza VLPs induced strong antibody responses against HA that inhibited hemagglutination by influenza virus, similar to IIV vaccines. Compared to young mice, antibody responses in aged mice immunized with a low dose of either influenza VLPs or IIV vaccines exhibited markedly reduced avidity for HA. However, immunization of aged mice with a high dose of influenza VLPs induced antibody responses with high avidity similar to those in young mice. Furthermore, all vaccinated animals survived a lethal challenge by a mouse-adapted influenza virus (A/PR/8/34), indicating that influenza VLPs are highly efficacious for protection against influenza virus infection in both young and aged mice. (C) 2009 Elsevier B.V. All rights reserved.
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