Cloning of a 9-cis-epoxycarotenoid dioxygenase gene and the responses of Caragana korshinskii to a variety of abiotic stresses

文献类型： 外文期刊

第一作者： Wang, Xuemin

作者： Wang, Xuemin;Wang, Zan;Dong, Jie;Wang, Meizhen;Gao, Hongwen

作者机构：

关键词： abscisic acid;Caragana korshinskii;cloning;9-cis-epoxycarotenoid dioxygenase;stress tolerance

期刊名称：GENES & GENETIC SYSTEMS （ 影响因子：1.517； 五年影响因子：1.434 ）

ISSN： 1341-7568

年卷期： 2009 年 84 卷 6 期

页码：

收录情况： SCI

摘要： Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses. Oxidative cleavage of cis-epoxycarotenoids catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED) is the main regulatory step in the biosynthesis of ABA in higher plants. Using RACE technology, a full-length cDNA-encoding NCED gene was isolated and characterized from the leaves of Caragana korshinskii (Peashrub). The 2442-bp full-length CkNCED1 had a 1818-bp ORF, which encodes a peptide of 605 amino acids. The deduced amino acid sequence of CkNCED1 protein shared high identity with other NCEDs. Southern blot analysis revealed that the gene CkNCED1 was a single copy in the genome of C. korshinskii. When C. korshinskii plants were exposed to a water deficit, ABA accumulation was followed by large increases in CkNCED1 mRNA in leaves and sterns, but only a moderate increase in the roots. Conversely, rehydration of stressed leaves caused a rapid decrease in CkNCED1 mRNA and ABA levels. RTPCR and Quantitative real-time PCR analysis showed that salt stress rapidly induced the strong expression of CkNCED1 in leaves and roots of C. korshinskii, as well as ABA accumulation. The expression of CkNCED1 and ABA accumulation was also induced by cold stress and the application of exogenous ABA. Taken together, these results suggest that CkNCED1 likely plays a primary role in the biosynthesis of ABA in C. korshinskii.

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