文献类型: 外文期刊
第一作者: Liu, Li
作者: Liu, Li;Wang, Aili;Ma, Junhong;Lan, Ping;Ma, Wujun;Liu, Li;Xia, Xianchun;He, Zhonghu;Wang, Aili;Yan, Yueming;He, Zhonghu;Bekes, Frank;Appels, Rudi
作者机构:
关键词: Triticum aestivum L.;Quality;SDS-PAGE;MALDI-TOF-MS;HMW-GS
期刊名称:JOURNAL OF CEREAL SCIENCE ( 影响因子:3.616; 五年影响因子:3.891 )
ISSN: 0733-5210
年卷期: 2009 年 50 卷 2 期
页码:
收录情况: SCI
摘要: High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7(OE), 7(OE)+8, 7(OE)+8a*, 7(OE)+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7(OE), 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki. (C) 2009 Elsevier Ltd. All rights reserved.
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