A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding

文献类型: 外文期刊

第一作者: Liu, Li

作者: Liu, Li;Wang, Aili;Ma, Junhong;Lan, Ping;Ma, Wujun;Liu, Li;Xia, Xianchun;He, Zhonghu;Wang, Aili;Yan, Yueming;He, Zhonghu;Bekes, Frank;Appels, Rudi

作者机构:

关键词: Triticum aestivum L.;Quality;SDS-PAGE;MALDI-TOF-MS;HMW-GS

期刊名称:JOURNAL OF CEREAL SCIENCE ( 影响因子:3.616; 五年影响因子:3.891 )

ISSN: 0733-5210

年卷期: 2009 年 50 卷 2 期

页码:

收录情况: SCI

摘要: High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7(OE), 7(OE)+8, 7(OE)+8a*, 7(OE)+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7(OE), 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki. (C) 2009 Elsevier Ltd. All rights reserved.

分类号:

  • 相关文献

[1]Development of two multiplex PCR assays targeting improvement of bread-making and noodle qualities in common wheat. Zhang, X. K.,Liu, L.,He, Z. H.,Sun, D. J.,He, X. Y.,Xu, Z. H.,Zhang, P. P.,Chen, F.,Xia, X. C.,Zhang, X. K.,He, Z. H.. 2008

[2]Comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars. Ma, Wujun,Liu, Wan,Gao, Xuan,Wang, Ke,Wang, Shunli,Yan, Yueming,Zhang, Yanzhen,Zhang, Yong,He, Zhonghu,Zhang, Yong,He, Zhonghu. 2012

[3]Molecular detection of high- and low-molecular-weight glutenin subunit genes in common wheat cultivars from 20 countries using allele-specific markers. H. Jin,J. Yan,R. J. Peña,X. C. Xia,A. Morgounov,L. M. Han,Y. Zhang,Z. H. He. 2011

[4]Quality differences between NILs of wheat variety Long 97-586 possessing HMW-GS 7+8 and 7. Zhang LiLi,Zhang YanBin,Li JiLin,Zhang LiLi,Zhang YanBin,Zhao HaiBin,Song QingJie,Yu HaiYang,Zhang ChunLi,Xin WenLi,Xiao ZhiMin. 2010

[5]Effects of Allelic Variation in Glutenin Subunits and Gliadins on Baking-quality in Near-isogenic Lines of Common Wheat cv. Longmai 19. Song, W. F.,Ren, Z. Y.,Zhang, Y. B.,Zhao, H. B.,Song, Q. J.,Zhang, C. L.,Xin, W. L.,Xiao, Z. M.,Song, W. F.,Ren, Z. Y.,Zhang, Y. B.,Li, J. L.,Guo, C. H.,Lv, X. B..

[6]Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae. Zhao, Xiangjie,Li, Lin,Li, Bing,Zhao, Xiangjie,Kuang, Zheshi,Luo, Guoqing,Zhao, Xiangjie. 2015

[7]Proteomic Analysis of Osmotic Stress-Responsive Proteins in Sugarcane Leaves. Li, Yang-Rui,Qiu, Li-Hang,Huang, Xing,Zhou, Gui,Zou, Cheng-Lin,Huang, Li-Ping,Li, Yang-Rui,Srivastava, Manoj Kumar.

[8]Genetic Polymorphisms of Mc4R and IGF2 Gene Association with Feed Conversion Efficiency Traits in Beef Cattle. Du, Xin-hua,Chen, Cui,Yuan, Zheng-rong,Zhang, Li-min,Chen, Xiao-jie,Wang, Yan-hui,Gao, Xue,Zhang, Lu-pei,Gao, Hui-jiang,Li, Jun-ya,Xu, Shang-zhong. 2013

[9]Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS. Peng, Yanchun,Yu, Kan,Sun, Dongfa,Peng, Yanchun,Yu, Zitong,Islam, Shahidul,Zhang, Yujuan,Wang, Xiaolong,Ma, Wujun,Peng, Yanchun,Yu, Zitong,Islam, Shahidul,Zhang, Yujuan,Wang, Xiaolong,Ma, Wujun,Lei, Zhensheng,Sun, Dongfa.

[10]Genetic diversity analysis of faba bean (Vicia faba L.) germplasms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Hou, W. W.,Shi, J. B.,Liu, Y. J.,Zhang, X. J.. 2015

[11]Identification of SNPs and development of allelic specific PCR markers for high molecular weight glutenin subunit D(t)x1.5 from Aegilops tauschii through sequence characterization. Yang, WY,Zhang, WJ,Lu, BR. 2005

[12]Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library. Tao, Bo,Shao, Bai-Hui,Qiao, Yu-Xin,Wang, Xiao-Qin,Chang, Shu-Jun,Qiu, Li-Juan. 2017

[13]Characterization of insecticidal crystal protein cry gene of Bacillus thuringiensis from soil of Sichuan Basin, China and cloning of novel haplotypes cry gene. Zhu, Jun,Tang, Jie,Li, Yunyan,Zheng, Aiping,Li, Ping,Tan, Furong,Zheng, Aiping,Zhu, Jun,Tang, Jie,Li, Yunyan,Li, Ping.

[14]Impact of vacuum mixing on protein composition and secondary structure of noodle dough. Zhang, Yingquan,Wu, Liang,Kong, Yan,Wei, Yimin,Liu, Rui,Sun, Junmao,Xing, Yanan.

[15]Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats. Liu, L,He, ZH,Yan, J,Zhang, Y,Xia, XC,Pena, RJ. 2005

[16]Study on the Change of Muscle Proteins During the Half-Dried Salt-Cured Silver Carp (Hypophthalmichthys molitrix) Processing. Gao, Ruichang,Yuan, Li,Su, Li,Huang, Xingyi,Yu, Gang,Li, Laihao.

[17]Analysis of ginsenoside content, functional genes involved in ginsenosides biosynthesis, and activities of antioxidant enzymes in Panax quinquefolium L. adventitious roots by fungal elicitors. Li, Jinxin,Li, Hongfa,Wang, Juan,Liu, Shujie,Li, Jianli,Liu, Dahui.

[18]Quality of synthetic hexaploid wheat containing null alleles at Glu-A1 and Glu-B1 loci. Dai, Shoufen,Liu, Dengcai,Jiang, Jiaqi,Wei, Yuming,Wu, Bihua,Lan, Xiujin,Zheng, Youliang,Yan, Zehong,Pu, Zhien,Pu, Zongjun. 2013

[19]Generation of transgenic wheat lines with altered expression levels of 1Dx5 high-molecular weight glutenin subunit by RNA interference. Yue, S. J.,Li, Y. W.,Zhu, Y. F.,Chen, Y.,Jia, X.,Li, H.,Yue, S. J.. 2008

[20]Differentiation of the high molecular weight glutenin subunit D-t x2.1 of Aegilops tauschii indicated by partial sequences of its encoding gene and SSR markers. Yang, WY,Lu, BR. 2005

作者其他论文 更多>>