Variation of B Chromosome Associated with Tissue Culture in Wheat-rye Cross

文献类型: 外文期刊

第一作者: Li, Hongjie

作者: Li, Hongjie;Tian, Bohong

作者机构:

关键词: amphiploid;B chromosome;genomic in situ hybridization;Secale cereale;tissue culture;Triticum aestivum

期刊名称:JOURNAL OF INTEGRATIVE PLANT BIOLOGY ( 影响因子:7.061; 五年影响因子:6.002 )

ISSN: 1672-9072

年卷期: 2009 年 51 卷 9 期

页码:

收录情况: SCI

摘要: In vitro variation of B chromosomes was studied by examining the callus cells derived from the immature embryos from a cross of Chinese Spring wheat (Triticum aestivum L.) and Fin 7416 rye (Secale cereale L.) carrying two B chromosomes. In 40-d-old callus cells, the numbers of B chromosomes ranged from one to four in 65.6% of the cells observed. The distribution of B chromosome numbers was associated with the ploidy levels of the normal chromosomes (A chromosomes). The frequency of the cells with high numbers of B chromosomes (i.e., three or four B chromosomes) in the amphiploid cells with 56 A chromosomes was greater than those in the haploid cells with 28 A chromosomes. Although structural changes in the rye A chromosomes were observed, cytological observation and genomic in situ hybridization demonstrated that the rye B chromosomes were conserved in morphological appearance following tissue culture.

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