Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008

文献类型： 外文期刊

第一作者： Zhou, Yan-Jun

作者： Zhou, Yan-Jun;Yu, Hai;Li, Guo-Xin;Yan, Li-Ping;Xu, Ao-Tian;Wang, Ya-Xin;Zhang, Shan-Rui;Tong, Guang-Zhi;Tian, Zhi-Jun;Hao, Xiao-Fang;Peng, Jin-Mei;An, Tong-Qing;Wei, Tian-Chao;Cai, Xue-Hui;Feng, Li;Li, Xi;Zhang, Gui-Hong;Zhou, Lun-Jiang

作者机构：

关键词： PRRSV;Genetic diversity;ORF5;China

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2009 年 144 卷 1-2 期

页码：

收录情况： SCI

摘要： Since April 2006, swine herds have experienced the outbreaks of a highly pathogenic porcine reproductive and respiratory syndrome (PRRS) in China. To explore the possible mechanism of the emergence of the highly pathogenic PRRS and more fully understand the extent of genetic diversity of PRRSV in China, we analyzed the ORF5 gene sequences of 159 representative PRRSV isolates in 16 provinces from 2006 to 2008. Sequence and phylogenetic analyses showed that all these 159 isolates belonged to the North American genotype and were further divided into six subgenotypes; 140 of 159 isolates were closely related to the highly pathogenic PRRSV with 98.5-100% nucleotide and 98.3-100% amino acid sequence identities and belonged to Subgenotype I; and 3, 8,4,3, 1 of 159 isolates were part of Subgenotypes II-VI, respectively. Amino acid analysis of the GP5 protein revealed that all the isolates in Subgenotypes I-III were found to be highly variable in the primary neutralizing epitope; most of the isolates in Subgenotypes I and IV had more glycosylation sites than those in Subgenotypes II, III, V and VI; and 1, 5, and 9 unique amino acid mutations were observed in Subgenotypes I, IV and VI, respectively. In conclusion, our study provides the evidence of coexistence of six different subgenotype isolates in pigs in China from 2006 to 2008, and emphasizes the importance of reinforcing PRRSV surveillance, especially after the emergence of highly pathogenic PRRS in China. (C) 2009 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Cloning and Sequence Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus of the ZJ Strain. Hao, Baocheng,Niu, Dengyuan,Xing, Xiaoyong,Xiang, Haitao,Wen, Fengqin,Fu, Xiaoping,Liang, Jianping,Hu, Yonghao,Niu, Dengyuan,Liang, Jianping. 2014

[2]GENETIC DIVERSITY ANALYSIS OF THE ORF5 GENE IN PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS SAMPLES FROM SOUTH CHINA. Cao, Zong-Xi,Jiao, Pei-Rong,Qin, Hong-Yang,Kong, Liu-Wu,Pan, Quan-Hui,He, Yi-Min,Zhang, Gui-Hong,Cao, Zong-Xi,Huang, Yu-Mao. 2012

[3]Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA. Meng, Xian Hong,Wang, Qing Yin,Liu, Ping,Kong, Jie,Meng, Xian Hong,Jang, In Kwon.

[4]Characterization of two newly emerged isolates of porcine reproductive and respiratory syndrome virus from Northeast China in 2013. Leng, Chao-liang,Sun, Long,Zhang, Gui-hong,Tong, Guang-zhi,Leng, Chao-liang,Tian, Zhi-jun,Zhang, Wu-chao,Zhang, Hong-liang,Zhai, Hong-yue,An, Tong-qing,Peng, Jin-mei,Ye, Chao,Wang, Qian,Sun, Yan,Li, Lin,Zhao, Hong-yuan,Chang, Dan,Cai, Xue-hui,Tong, Guang-zhi,Tong, Guang-zhi.

[5]Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain. Jiang, Yi-feng,Xia, Tian-qi,Zhou, Yan-jun,Yu, Ling-xue,Yang, Shen,Huang, Qin-feng,Li, Li-wei,Gao, Fei,Qu, Ze-hui,Tong, Wu,Tong, Guang-zhi,Jiang, Yi-feng,Zhou, Yan-jun,Tong, Guang-zhi.

[6]Characterization of two novel porcine reproductive and respiratory syndrome virus isolates with deletions in the GP2 gene. Chen, Jia-zeng,Peng, Jin-mei,Bai, Yun,Wang, Qian,Liu, Yi-min,Zhang, Qiu-yue,Chang, Dan,Zhang, Wu-chao,Zhao, Hong-yuan,Ye, Chao,An, Tong-qing,Cai, Xue-hui,Tian, Zhi-jun,Tong, Guang-zhi.

[7]Complete genomic characterization of a porcine reproductive and respiratory syndrome virus isolate in Xinjiang province of China. Xia, Jun,Du, Wei,Liu, Yanfeng,Yan, Genqiang,Xia, Jun,Chen, Shijun,Huang, Jion,Ma, Wenge,Yang, Xueyun.

[8]Construction of a Genetic Linkage Map and Mapping of a Female-Specific DNA Marker in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Liao, Xiaolin,Ma, Hong-Yu,Xu, Gen-Bo,Shao, Chang-Wei,Tian, Yong-Sheng,Ji, Xiang-Shan,Yang, Jing-Feng,Chen, Song-Lin.

[9]Genetic variation in the invasive process of Bursaphelenchus xylophilus (Aphelenchida : Aphelenchoididae) and its possible spread routes in China. Cheng, X-Y,Cheng, F-X,Xu, R-M,Xie, B-Y. 2008

[10]Population structure of Haemonchus contortus from seven geographical regions in China, determined on the basis of microsatellite markers. Yin, Fangyuan,Gasser, Robin B.,Li, Facai,Yang, Xin,Zhou, Yanqin,Zhao, Junlong,Fang, Rui,Hu, Min,Yin, Fangyuan,Li, Facai,Gasser, Robin B.,Bao, Min,Huang, Weiyi,Zou, Fengcai,Zhao, Guanghui,Wang, Chunren. 2016

[11]A preliminary comparative evaluation of genetic diversity between Chinese and Japanese wild soybean (Glycine soja) germplasm pools using SSR markers. Wang, Ke-Jing,Takahata, Yoshihito. 2007

[12]Analysis of genetic diversity among different geographical populations and determination of biotypes of Bemisia tabaci in China. Zhang, LP,Zhang, YJ,Zhang, WJ,Wu, QJ,Xu, BY,Chu, D. 2005

[13]Isolation and characterization of twenty-six microsatellite loci for the tetraploid fish Dabry's sturgeon (Acipenser dabryanus). Zhang, Shu-Huan,Luo, Hui,Du, Hao,Wang, Deng-Qiang,Wei, Qi-Wei,Zhang, Shu-Huan,Luo, Hui,Du, Hao,Wang, Deng-Qiang,Wei, Qi-Wei. 2013

[14]Population Genetic Analysis of Theileria annulata from Six Geographical Regions in China, Determined on the Basis of Micro- and Mini-satellite Markers. Yin, Fangyuan,Liu, Zhijie,Liu, Junlong,Liu, Aihong,Li, Youquan,Liu, Guangyuan,Luol, Jianxun,Guan, Guiquan,Yin, Hong,Salih, Diaeldin A.,Yin, Hong. 2018

[15]Analysis of Genetic Structure and Diversity of Chai Chicken Breed Using Microsatellite Markers. Zhao, Zhen-Hua,Li, Shou-Feng,Huang, Hua-Yun,Xi, Jian-Zhong,Jia, Qin. 2010

[16]The Genetic Diversity of Hainan Black Goat by Capillary Gel Electrophoresis with Microsatellite Markers in Tropical Area of China. Zhou, H. L.,Guan, S.,Hou, G. Y.,Wang, D. J.,Xu, T. S.,Wang, D. F.,Shi, L. G.,Rong, G.,Li, M.. 2012

[17]Genetic diversity and phylogenetic analysis of glycoprotein GP85 of ALV-J isolates from Mainland China between 1999 and 2010: Coexistence of two extremely different subgroups in layers. Pan, Wei,Gao, Yulong,Qin, Litin,Ni, Wei,Liu, Zaisi,Yun, Binglin,Wang, Yongqiang,Qi, Xiaole,Gao, Honglei,Wang, Xiaomei.

[18]Genome composition and genetic diversity of Musa germplasm from China revealed by PCR-RFLP and SSR markers. Ning, Shu-Ping,Xu, Lin-Bing,Lu, Yan,Huang, Bing-Zhi,Ge, Xue-Jun. 2007

[19]Molecular characterization of porcine circovirus-like virus P1 in eastern China. Wen, Libin,Jiao, Fangfang,Zhang, Dan,Mao, Aihua,Liu, Chuanmin,Xie, Jianping,He, Kongwang,Wen, Libin,Jiao, Fangfang,Zhang, Dan,Mao, Aihua,Liu, Chuanmin,Xie, Jianping,He, Kongwang,Li, Yiming.

[20]Retrotransposon-microsatellite amplified polymorphism, an electrophoretic approach for studying genetic variability among Schistosoma japonicum geographical isolates. Li, Juan,Zhao, Guang-Hui,Zhou, Dong-Hui,Zhu, Xing-Quan,Li, Juan,Li, Juan,Li, Xiao-Yan,Zhao, Guang-Hui,Sugiyama, Hiromu,Zou, Feng-Cai,Zhu, Xing-Quan,Li, Hai-Long,Ai, Lin.

作者其他论文 更多>>

微信小程序