Isolation and biochemical characterization of two lipases from a metagenomic library of China Holstein cow rumen

文献类型： 外文期刊

第一作者： Liu, Kailang

作者： Liu, Kailang;Wang, Jiaqi;Bu, Dengpan;Zhao, Shengguo;Yu, Ping;Li, Dan;McSweeney, Chris

作者机构：

关键词： Metagenomics;Lipase;Culture-independent;Rumen

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2009 年 385 卷 4 期

页码：

收录情况： SCI

摘要： Two novel lipase genes RlipE1 and RlipE2 which en coded 361- and 265-amino acid peptides, respectively, were recovered from a metagenomic library of the rumen microbiota of Chinese Holstein cows. A BLAST search revealed a high similarity (90%) between RlipE2 and a carboxylesterase from Thermosinus carboxydivorans Nor1, while there was a low similarity (below 50%) between RlipE1 and other lipases. Phylogenetic analysis indicated that RlipE2 clustered with the lipolytic enzymes from family V while RlipE1 Clustered with six other Putative bacterial lipases which might Constitute a new subfamily. The recombinant lipases were thermally unstable and retained 60% activity over a pH range of 6.5-8.5. Substrate specificity assay indicated that both enzymes had higher hydrolytic activity toward laurate (C-12), palmitate (C-16) and stearate (C-18). The novel phylogenetic affiliation and high specificity of both enzymes for long-chain fatty acid make them interesting targets for manipulation Of rumen lipid metabolism. (c) 2009 Elsevier Inc. All rights reserved.

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