Microvalves actuated sandwich immunoassay on an integrated microfluidic system

文献类型： 外文期刊

第一作者： Gao, Xinghua

作者： Gao, Xinghua;Jiang, Lei;Qin, Jianhua;Lin, Bingcheng;Su, Xiaoou

作者机构：

关键词： Human immunoglobulin G;Immunoassay;Integration;Microfluidics;Microvalve

期刊名称：ELECTROPHORESIS （ 影响因子：3.535； 五年影响因子：2.731 ）

ISSN： 0173-0835

年卷期： 2009 年 30 卷 14 期

页码：

收录情况： SCI

摘要： In this study, we developed an integrated and automatic microfluidic immunoassay system, which comprised a multi-layer immunoassay microfluidic chip and a rotary confocal LIF scanner. The normally closed elastomeric microvalves were integrated on the multi-layer microfluidic chip to realize flexible reagents delivery for the rapid immunoassay. The various operations of immunoassay including coating, blocking and washing could be performed on a single chip with reduced reagents consumption and analysis time. The homemade rotary LIF scanner was able to detect the signals from the multichannels sequentially in a short period of time, enabling the potential capability for high-throughput immunoassay. The performance of this system was demonstrated by the sandwich immunoassay of human immunoglobulin G with a LOD < 10 ng/mL (S/N = 3). The linear range was from 500 ng/mL to 100 mu g/mL. This system has also been applied to the measurement of human immunoglobulin G from 15 real samples, demonstrating the potential of this platform for biomedical applications.

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