Identification of Secreted Antigen 3 from Babesia gibsoni

文献类型： 外文期刊

第一作者： Jia, Honglin

作者： Jia, Honglin;Terkawi, M. Alaa;Aboge, Gabriel Oluga;Goo, Youn-Kyoung;Ma, Liqing;Zhou, Jinlin;Nishikawa, Yoshifumi;Igarashi, Ikuo;Fujisaki, Kozo;Xuan, Xuenan;Ma, Liqing;Zhou, Jinlin

作者机构：

期刊名称：CLINICAL AND VACCINE IMMUNOLOGY （ 影响因子：3.233； 五年影响因子：2.562 ）

ISSN： 1556-6811

年卷期： 2009 年 16 卷 6 期

页码：

收录情况： SCI

摘要： A cDNA expression library of Babesia gibsoni was screened with the serum collected from a dog experimentally infected with B. gibsoni. A novel antigen sharing homology with secreted antigen 1 of B. gibsoni was isolated. The genomic analysis indicated that the BgSA3 gene exists as multicopies in the genome of B. gibsoni. The putative peptide encoded by the BgSA3 gene showed some characteristics of secreted proteins. The serum raised in mice immunized with the recombinant BgSA3 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 70 kDa. Moreover, a sandwich enzyme-linked immunosorbent assay with anti-BgSA3 antibodies could detect the circulating BgSA3 in the blood plasma of dogs experimentally infected with B. gibsoni. The identification of BgSA3 provided a useful target for the development of a diagnostic test for detecting specific antibodies and circulating antigens.

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