A multiplex PCR for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus in clinical specimens

文献类型: 外文期刊

第一作者: Yue, Fengxiong

作者: Yue, Fengxiong;Cui, Shangjin;Zhang, Chaofan;Yoon, Kyoung-Jin

作者机构:

关键词: Multiplex PCR (mPCR);Porcine circovirus type2 (PCV2);Porcine parvovirus (PPV);Porcine pseudorabies virus (PRV);Porcine reproductive and respiratory syndrome virus (PRRSV)

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

ISSN: 0920-8569

年卷期: 2009 年 38 卷 3 期

页码:

收录情况: SCI

摘要: A multiplex PCR (mPCR) assay was developed and evaluated for its ability to simultaneously detect multiple viral infections of swine. Specific primers were designed for each of the following four DNA or RNA viruses: porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), pseudorabies virus (PRV), and porcine reproductive and respiratory syndrome virus (PRRSV). Each target produced a specific amplicon with a size of 353 bp (PCV2), 271 bp (PPV), 194 bp (PRV), or 434 bp (PRRSV). The assay was sensitive and specific in detecting each target agent in composite cell cultures and clinical specimens. Results from mPCR were confirmed by PCR for individual viruses and by virus isolation. In conclusion, the mPCR has the potential to be useful for routine molecular diagnosis and epidemiology.

分类号:

  • 相关文献

[1]Novel multiplex PCR assay using locked nucleic acid (LNA)-based universal primers for the simultaneous detection of five swine viruses. Chen, Ru,Qiu, Yang,Gao, Xiao-Bo,Yu, Xiao-Lu,Song, Chang-Xu.

[2]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[3]Rapid and specific detection of porcine parvovirus using real-time PCR and High Resolution Melting (HRM) analysis. Yu, Hai-Qiong,Lin, Zhi-Xiong,Cai, Xian-Quan,Yue, Qiao-Yun,Li, Rong,Li, Xiang-Li,Zhu, Xing-Quan. 2015

[4]The prevalent status and genetic diversity of porcine reproductive and respiratory syndrome virus in China: a molecular epidemiological perspective. Guo, Zhenhua,Chen, Xin-xin,Li, Rui,Qiao, Songlin,Zhang, Gaiping,Zhang, Gaiping,Zhang, Gaiping. 2018

[5]Porcine reproductive and respiratory syndrome virus attachment is mediated by the N-terminal domain of the sialoadhesin receptor. Tong, Guang-Zhi,An, Tong-Qing,Tian, Zhi-Jun,He, Yun-Xia,Xiao, Yan,Jiang, Yi-Feng,Peng, Jin-Mei,Zhou, Yan-Jun,Tong, Guang-Zhi,An, Tong-Qing,Liu, Di.

[6]Amino acid at position 176 was essential for porcine reproductive and respiratory syndrome virus (PRRSV) non-structural protein 1 alpha (nsp1 alpha) as an inhibitor to the induction of IFN-beta. Shi, Xibao,Chen, Jing,Xing, Guangxu,Zhang, Gaiping,Shi, Xibao,Chen, Jing,Xing, Guangxu,Hu, Xiaofei,Zhi, Yubao,Guo, Junqing,Wang, Li,Qiao, Songlin,Lu, Qingxia,Zhang, Gaiping,Zhang, Xiaozhuan.

[7]Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Inhibits RNA-Mediated Gene Silencing by Targeting Ago-2. Chen, Jing,Zhang, Gaiping,Chen, Jing,Shi, Xibao,Wang, Li,Luo, Jun,Xing, Guangxu,Deng, Ruiguang,Zhang, Gaiping,Shi, Xibao,Zhang, Xiaozhuan,Yang, Hong,Li, Jinting,Wang, Aiping,Zhang, Gaiping. 2015

[8]Porcine reproductive and respiratory syndrome virus (PRRSV) could be sensed by professional beta interferon-producing system and had mechanisms to inhibit this action in MARC-145 cells. Shi, Xibao,Wang, Li,Zhi, Yubao,Xing, Guangxu,Zhao, Dong,Deng, Ruiguang,Zhang, Gaiping. 2010

[9]Endoribonuclease activities of porcine reproductive and respiratory syndrome virus nsp11 was essential for nsp11 to inhibit IFN-beta induction. Shi, Xibao,Wang, Li,Li, Xuewu,Zhang, Gaiping,Guo, Junqing,Zhao, Dong,Chai, Shujun,Deng, Ruiguang. 2011

[10]Downregulation of protein kinase PKR activation by porcine reproductive and respiratory syndrome virus at its early stage infection. Xiao, Yueqiang,Ma, Zexu,Wang, Rong,Yang, Liping,Nan, Yuchen,Zhang, Yan-Jin,Xiao, Yueqiang,Ma, Zexu,Wang, Rong,Yang, Liping,Nan, Yuchen,Zhang, Yan-Jin,Xiao, Yueqiang.

[11]Antibody-dependent enhancement of PRRSV infection down-modulates TNF-alpha and IFN-beta transcription in macrophages. Bao, Dengke,Wan, Bo,Wang, Yinbiao,Zhang, Gaiping,Bao, Dengke,Wang, Rui,Qiao, Songlin,Wan, Bo,Wang, Yinbiao,Liu, Mingyang,Shi, Xibao,Guo, Juiqing,Zhang, Gaiping.

[12]Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFN gamma. Xue, QN,Zhao, YG,Zhou, YJ,Qiu, HJ,Wang, YF,Wu, DL,Tian, ZJ,Tong, GZ. 2004

[13]Porcine Reproductive and Respiratory Syndrome Virus Antagonizes JAK/STAT3 Signaling via nsp5, Which Induces STAT3 Degradation. Zhang, Yan-Jin,Wang, Rong,Xiao, Yueqiang,Nan, Yuchen,Wang, Yu.

作者其他论文 更多>>