A multiplex PCR for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus in clinical specimens
文献类型: 外文期刊
第一作者: Yue, Fengxiong
作者: Yue, Fengxiong;Cui, Shangjin;Zhang, Chaofan;Yoon, Kyoung-Jin
作者机构:
关键词: Multiplex PCR (mPCR);Porcine circovirus type2 (PCV2);Porcine parvovirus (PPV);Porcine pseudorabies virus (PRV);Porcine reproductive and respiratory syndrome virus (PRRSV)
期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )
ISSN: 0920-8569
年卷期: 2009 年 38 卷 3 期
页码:
收录情况: SCI
摘要: A multiplex PCR (mPCR) assay was developed and evaluated for its ability to simultaneously detect multiple viral infections of swine. Specific primers were designed for each of the following four DNA or RNA viruses: porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), pseudorabies virus (PRV), and porcine reproductive and respiratory syndrome virus (PRRSV). Each target produced a specific amplicon with a size of 353 bp (PCV2), 271 bp (PPV), 194 bp (PRV), or 434 bp (PRRSV). The assay was sensitive and specific in detecting each target agent in composite cell cultures and clinical specimens. Results from mPCR were confirmed by PCR for individual viruses and by virus isolation. In conclusion, the mPCR has the potential to be useful for routine molecular diagnosis and epidemiology.
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