A xylanase with high pH stability from Streptomyces sp S27 and its carbohydrate-binding module with/without linker-region-truncated versions

文献类型： 外文期刊

第一作者： Li, Ning

作者： Li, Ning;Shi, Pengjun;Yang, Peilong;Wang, Yaru;Luo, Huiying;Bai, Yingguo;Zhou, Zhigang;Yao, Bin

作者机构：

关键词： Streptomyces sp S27;Xylanase;Carbohydrate-binding module;Linker;Stability

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2009 年 83 卷 1 期

页码：

收录情况： SCI

摘要： A xylanase gene, xynAS27, was isolated from a genomic library of Streptomyces sp. S27. The full-length gene consists of 1,434 bp and encodes 477 amino acids, including a putative signal peptide of 41 residues at its N-terminus. The mature xylanase comprises two functional domains, a family 10 glycoside hydrolase, and a family 13 carbohydrate-binding module (CBM), which were joined by a short Gly/Pro-rich linker region. The intact, the CBM-truncated and the CBM-linker-truncated versions of the mature proteins were expressed in Escherichia coli BL21 (DE3), purified to electrophoretic homogeneity and subsequently characterized. XynAS27 showed high pH stability over the pH range 2.2-12.0. XynAS27 may be a compelling tool for the food industry because it generates xylobiose (85% w/w) as the main product of xylan hydrolysis. The truncated versions showed less pH and thermal stability, and less affinity and hydrolytic activity to insoluble substrate than the intact one. These results indicate that the CBM of XynAS27 plays a key role in the hydrolysis of insoluble substrate, and the CBM and linker region are also important for the enzyme stability, and the linker region contributes more.

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