A real-time PCR assay for quantification of the Y136F allele in the CYP51 gene associated with Blumeria graminis f.sp tritici resistance to sterol demethylase inhibitors

文献类型： 外文期刊

第一作者： Yang, Qianqian

作者： Yang, Qianqian;Ma, Zhonghua;Zhou, Yilin;Duan, Xiayu

作者机构：

关键词： Blumeria graminis f.sp tritici;Fungicide resistance;Real-time PCR;Sterol 14 alpha-demethylase inhibiting fungicide

期刊名称：CROP PROTECTION （ 影响因子：2.571； 五年影响因子：3.11 ）

ISSN： 0261-2194

年卷期： 2009 年 28 卷 5 期

页码：

收录情况： SCI

摘要： A single point mutation resulting in the replacement of TAT by TIT at codon 136 (Y136F) in the 14 alpha-demethylase (CYP51) gene was detected from triadimefon-resistant Blumeria graminis f.sp. tritici (Bgt) isolates collected from different locations in China. Based on this point mutation, a pair of PCR primers Bgt136-F + Bgt136-R was developed for the specific detection of Y136F mutation. Additionally, based on the sequence of introns of the CYP51 gene from Bgt, another pair of PCR primers Bgt-F + Bgt-R was developed for specific detection of Bgt. Using these two primer pairs, a real-time PCR method was developed for rapid quantification of DMI-resistant Bgt populations from field samples. (C) 2009 Elsevier Ltd. All rights reserved.

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