Identification of a virus-specific and conserved B-cell epitope on NS1 protein of Japanese encephalitis virus

文献类型： 外文期刊

第一作者： Tong, Guang-Zhi

作者： Tong, Guang-Zhi;Bin Wang;Hua, Rong-Hong;Tian, Zhi-Jun;Chen, Na-Sha;Zhao, Fu-Rong;Liu, Tian-Qiang;Wang, Yun-Feng;Tong, Guang-Zhi

作者机构：

关键词： Japanese encephalitis virus;Non-structural protein;Monoclonal antibody;Epitopes;Differential diagnosis

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2009 年 141 卷 1 期

页码：

收录情况： SCI

摘要： NS1 protein of Japanese encephalitis virus (JEV) is an important non-structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool, but the epitopes on NS1 of JEV have not been identified. For epitope mapping, in this study, a series of 51 partially overlapping fragments covering entire NS1 protein were expressed with a GST-tag and then screened by a monoclonal antibody (mAb). Through enzyme-linked immunosorbent assay (ELISA), linear epitope-containing fragment, the overlapping region of NS1-18 and NS1-19 (residues 145-152), was located. Then a set of peptides derived from that overlapping region with deletions were expressed and subjected to ELISA and Western blot for further mapping purpose. Results indicated that the motif of (146)EHARW(150) is the minimal unit of the linear epitope recognized by that monoclonal antibody (mAb). Western blot showed that this epitope could be recognized by JEV-positive serum from pigs. Furthermore, it was found that the epitope is highly conserved among JEV strains through sequence alignments analysis. Notably, none of the homologous regions on NS1 proteins of other flavivirus could react with the mAb when they were tested for cross-reactivity, suggesting the potential clinical application of this epitope in differential diagnosis. (C) 2009 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Expression of the major epitope regions of 2C integrated with the 3AB non-structural protein of foot-and-mouth disease virus and its potential for differentiating infected from vaccinated animals. Lu, Zengjun,Zhang, Xiaoli,Fu, Yuanfang,Cao, Yimei,Tian, Meina,Sun, Pu,Li, Dong,Liu, Zaixin,Xie, Qingge,Zhang, Xiaoli. 2010

[2]Development of a dot immunoblot method for differentiation of animals infected with foot-and-mouth disease virus from vaccinated animals using non-structural proteins expressed prokaryotically. Fu, Yuanfang,Cao, Yimei,Sun, Pu,Bao, Huifang,Bai, Xingwen,Li, Pinghua,Li, Dong,Lu, Zengjun,Liu, Zaixin. 2011

[3]Pig BVDV-2 non-structural protein (N-pro) links to cellular antiviral response in vitro. Tao, Jie,Liao, Jinhu,Wang, Jianye,Zhang, Xinjun,Zhang, Qian,Zhu, Liqian,Zhu, Guoqiang,Tao, Jie,Liao, Jinhu,Wang, Jianye,Zhang, Xinjun,Zhang, Qian,Zhu, Liqian,Zhu, Guoqiang,Tao, Jie,Liu, Huili,Zhang, Weiping.

[4]Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs. Yi, Li,Cheng, Shipeng,Xu, Hongli,Wang, Jianke,Cheng, Yuening,Yang, Shen,Luo, Bin. 2012

[5]A multiplex nested RT-PCR for the detection and differentiation of wild-type viruses from C-strain vaccine of classical swine fever virus. Li, Yan,Zhao, Jian-Jun,Li, Na,Shi, Zixue,Cheng, Dan,Zhu, Qing-Hu,Tu, Changchun,Tong, Guang-Zhi,Qiu, Hua-Ji. 2007

[6]Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis. Quan, Zhang,Haiming, Tan,Xiaoya, Cai,Weifeng, Yuan,Hong, Jia,Hongfei, Zhu.

[7]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[8]Differential Diagnosis of Antibody to Classical Swine Fever Virus Field Strain by ELISA with Recombinant E2 Proteins of Various Group CSFV. Qiu Chang-Qing,Hu Hui,Cao Xiao-An,Zhou Ji-Zhang,Lin Guo-Zhen,Hu Hui. 2008

[9]Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. Liu, Da-Fei,Chai, Hong-Liang,Yin, Xiu-Chen,Hua, Yu-Ping,Liu, Da-Fei,Liu, Chun-Guo,Tian, Jin,Zhang, Xiao-Zhan,Yang, Tian-Kuo,Zhang, Hong-Ying,Liu, Ming,Qu, Lian-Dong,Jiang, Yi-Tong.

[10]Evaluation of a real-time RT-PCR assay using minor groove binding probe for specific detection of Chinese wild-type classical swine fever virus. Wen, Guoyuan,Yang, Jun,Luo, Qingping,Liao, Yonghong,Zhang, Rongrong,Wang, Hongling,Ai, Diyun,Luo, Ling,Shao, Huabin,Zhang, Tao,Wen, Guoyuan,Hu, Zhibin,Song, Nianhua,Zhang, Tao. 2011

[11]Analysis of murine B-cell epitopes on bluetongue virus 12 nonstructural protein 1. Wang HaiXiu,Sun EnCheng,Xu QingYuan,Yang Tao,Zhang Qin,Feng YuFei,Li JunPing,Lv Shuang,Sun Liang,Sun Jing,Wu DongLai.

[12]Characterization of monoclonal antibodies that recognize the amino- and carboxy-terminal epitopes of the pseudorabies virus UL42 protein. Du, Wenjuan,Wang, Yiping,Huang, Liping,Wei, Yanwu,Chen, Dongjie,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

[13]In silico prediction and in vitro identification of bluetongue virus 4 VP5 protein B-cell epitopes. Sun, L.,Sun, E. C.,Yang, T.,Xu, Q. Y.,Feng, Y. F.,Li, J. P.,Sun, J.,Wang, W. S.,Wei, P.,Wu, D. L.,Sun, L.,Sun, E. C.,Yang, T.,Xu, Q. Y.,Feng, Y. F.,Li, J. P.,Sun, J.,Wang, W. S.,Wei, P..

[14]Identification of epitopes on nonstructural protein 7 of porcine reproductive and respiratory syndrome virus recognized by monoclonal antibodies using phage-display technology. Wang, Heng,Sun, Lingshuang,Ning, Zhangyong,Zhang, Guihong,Wang, Heng,Sun, Lingshuang,Ning, Zhangyong,Ji, Fangxiao,Cui, Jin,Zhang, Guihong,Liu, Rongchang,Zhang, Weidong.

[15]Identification of two novel BTV16-specific B cell epitopes using monoclonal antibodies against the VP2 protein. Wang, Wen-Shi,Sun, En-Cheng,Xu, Qing-Yuan,Yang, Tao,Qin, Yong-Li,Zhao, Jing,Feng, Yu-Fei,Li, Jun-Ping,Wei, Peng,Zhang, Cui-Yun,Wu, Dong-lai.

[16]Influence of Gamma Irradiation and Heat Treatment on the Immunogenicity of Five Epitopes of Pen a1. Mou, Hui,Gao, Mei-xu,Zhao, Jie,Zhao, Xin,Wang, Zhi-dong,Pan, Jia-rong.

[17]Review on the identification and role of Toxoplasma gondii antigenic epitopes. Wang, Yanhua,Wang, Guangxiang,Cai, Jianping,Yin, Hong.

[18]Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2. Ding, Peiyang,Li, Yafei,Zhou, Enmin,Zhang, Gaiping,Ding, Peiyang,Zhang, Teng,Li, Yafei,Teng, Man,Sun, Yaning,Liu, Xiao,Chai, Shujun,Jin, Qianyue,Zhang, Gaiping,Zhang, Teng,Liu, Xiao,Zhang, Gaiping,Jin, Qianyue,Zhang, Gaiping. 2017

[19]Identification of three novel linear B-cell epitopes on the VP5 protein of BTV16. Wang, Wen-Shi,Sun, En-cheng,Liu, Ni-Hong,Yang, Tao,Xu, Qing-Yuan,Qin, Yong-Li,Zhao, Jing,Feng, Yu-Fei,Li, Jun-Ping,Wei, Peng,Zhang, Cui-Yun,Wu, Dong-lai. 2013

[20]Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken. Tan, Lei,Liao, Ying,Fan, Jin,Zhang, Yuqiang,Mao, Xiang,Sun, Yingjie,Song, Cuiping,Qiu, Xusheng,Meng, Chunchun,Ding, Chan,Ding, Chan.

作者其他论文 更多>>

微信小程序