Directed differentiation of chick embryonic germ cells into neural cells using retinoic acid induction in vitro

文献类型： 外文期刊

第一作者： Du, Li-xin

作者： Du, Li-xin;Wang, Juan;Pan, Xiao-hong;Niu, Xin-hua;Jiao, Fei;Xie, Shu-yang;Li, Zun-ling

作者机构：

关键词： Chick;Embryonic germ cell;Primordial germ cell;Differentiation;Retinoic acid

期刊名称：JOURNAL OF NEUROSCIENCE METHODS （ 影响因子：2.39； 五年影响因子：3.183 ）

ISSN： 0165-0270

年卷期： 2009 年 177 卷 1 期

页码：

收录情况： SCI

摘要： Embryonic germ cells (EG) are undifferentiated stem cells isolated from cultured primordial germ cells (PGC). Like embryonic stem cells (ES), EG are also capable of proliferation and self-renewal and have the capacity to differentiate in vitro into all cell types. To date, it has been proven that ES are capable of directed differentiation into neural precursors and progenies in mammals. However, similar studies on EG in mammals and other species are few. This investigation aimed to induce chick EG to differentiate into neural cells and compare the difference of efficiency between directed differentiation and spontaneous differentiation. EG were isolated and identified from 5.5-day chick gonadal PGC, incubated and passaged in conditioned medium. After the formation of embryoid bodies (EB), EB were grown in suspension and induced by retinoic acid (RA), using a protocol named 4-/4+, to make the formation of neurospheres and progenies. RT-PCR and immunocytochemistry analysis demonstrated that neural-specific markers can be detected after directed induction. Moreover, EG differentiated into neural lineage cells using 4-/4+ protocol much more efficiently than that in the spontaneous differentiation with fluorescence-activated cell sorting (FACS) analysis. The results revealed that RA can obviously promote the directed differentiation of chick EG into neural lineage. (C) 2008 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Directed Neural Differentiation of Duck Embryonic Germ Cells. Li, Lin-Feng,Bai, Chun-Yu,Gong, Xue-Lian,Guan, Wei-Jun,Ma, Yue-Hui,Li, Lin-Feng. 2011

[2]All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells. Gao, Yuhua,Wang, Kunfu,Zheng, Dong,Gao, Yuhua,Bai, Chunyu,Wang, Kunfu,Sun, Bo,Guan, Weijun.

[3]Derivation and characteristics of pluripotent embryonic germ cells in duck. Guan, W.,Wang, Y.,Chen, L.,Li, X.,Ma, Y.,Wang, Y.,Hou, L.,Yue, W.. 2010

[4]Chicken model for inducing primordial germ cells differentiating into motile sperms in vitro. Xiong, Hui,Li, Xiangchen,Hu, Pengfei,Guan, Weijun,Hu, Qingyun. 2017

[5]Retinoid Regulation of the Zebrafish cyp26a1 Promoter. Hu, Ping,Bao, Jie,Gao, Xiang,Zhao, Qingshun,Tian, Miao,Gu, Xingxing,Xing, Guangdong,Linney, Elwood. 2008

[6]Genetic variability assessed by microsatellites in the breeding populations of the shrimp Penaeus (Fenneropenaeus) chinensis in China. Zhang, Tianshi,Kong, Jie,Wang, Weiji,Wang, Qingyin,Zhang, Tianshi.

[7]Establishment of a pluripotent embryonic cell line from sea perch (Lateolabrax japonicus) embryos. Cher, SL,Sha, ZX,Ye, HQ.

[8]Suppression and restoration of primordial germ cell marker gene expression in channel catfish, Ictalurus punctatus, using knockdown constructs regulated by copper transport protein gene promoters: Potential for reversible transgenic sterilization. Su, Baofeng,Shang, Mei,Peatman, Eric,Perera, Dayan A.,Cheng, Qi,Li, Chao,Weng, Chia-Chen,Li, Ping,Liu, Zhanjiang,Dunham, Rex A.,Su, Baofeng,Shang, Mei,Grewe, Peter M.,Patil, Jawahar G.,Patil, Jawahar G.,Perera, Dayan A.,Li, Chao,Li, Ping.

[9]Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis. Yang Xiaoge,Ye Huan,Wei Qiwei,Yang Xiaoge,Yue Huamei,Ye Huan,Li Chuangju,Wei Qiwei,Yue Huamei,Li Chuangju,Wei Qiwei.

[10]Effects of species and cellular activity of oviductal epithelial cells on their dialogue with co-cultured mouse embryos. Tan, Xiu-Wen,Ma, Suo-Feng,Yu, Jian-Ning,Zhang, Xia,Lan, Guo-Cheng,Liu, Xin-Yong,Han, Zheng-Bin,Tan, Jing-He. 2007

[11]Activation of mammalian target of rapamycin signaling in skeletal muscle of neonatal chicks: Effects of dietary leucine and age. Deng, Huiling,Zheng, Aijuan,Liu, Guohua,Chang, Wenhuan,Zhang, Shu,Cai, Huiyi. 2014

[12]Effects of starter feeding time on body growth and viscera development of newly hatched chicks. Yang, Haiming,Wang, Zhiyue,Lu, Jian,Li, Wenzeng,Shi, Shourong. 2009

[13]Developmental increases in hypothalamic neuropeptide Y content with the embryonic age of meat- and layer-type chicks. Zhou, WD,Aoyama, M,Yoshizawa, F,Sugahara, K. 2006

[14]Expression and characterization of recombinant gallinacin-9 and gallinacin-8 in Escherichia coli. Ma, De-ying,Shan, An-shan,Liu, Sheng-wang,Han, Zong-xi,Li, Yi-jin.

[15]Characterization of a highly virulent and antimicrobial-resistant Acinetobacter baumannii strain isolated from diseased chicks in China. Liu, Dong,Liu, Zeng-Shan,Hu, Pan,Hui, Qi,Lu, Shi-Ying,Li, Yan-Song,Zou, De-Ying,Li, Zhao-Hui,Yan, Dong-Ming,Ding, Yan-Xia,Zhang, Yuan-Yuan,Zhou, Yu,Liu, Nan-Nan,Ren, Hong-Lin,Fu, Bao-Quan,Zou, De-Ying.

[16]The in ovo administration of L-trans pyrrolidine-2,4-dicarboxylic acid regulates small intestinal growth in chicks. Li, X. -G.,Sui, W. -G.,Yan, H. -C.,Jiang, Q. -Y.,Wang, X. -Q..

[17]MicroRNA-95 promotes myogenic differentiation by down-regulation of aminoacyl-tRNA synthase complex-interacting multifunctional protein 2. Li, Biao,Xie, Shanshan,Cai, Chunbo,Qian, Lili,Jiang, Shengwang,Ma, Dezun,Xiao, Gaojun,Gao, Ting,Cui, Wentao,Yang, Jinzeng. 2017

[18]Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies. Jin, C,Wang, PS,Xia, YM,Xu, M,Pei, SJ. 2005

[19]Isolation and Characterization of Mesenchymal Stem Cells Derived from Duck Embryo Liver. Wang, Wenjie,Guan, Weijun,Ma, Yuehui,Huang, Hongmei,Li, Qian,Wang, KunFu,Chen, Jia. 2017

[20]Klf7 modulates the differentiation and proliferation of chicken preadipocyte. Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Zhang, Zhiwei,Wang, Haixia,Sun, Yingning,Li, Hui,Wang, Ning,Sun, Yingning. 2013

作者其他论文 更多>>

微信小程序