Understanding Haemophilus parasuis infection in porcine spleen through a transcriptomics approach

文献类型： 外文期刊

第一作者： Chen, Hongbo

作者： Chen, Hongbo;Li, Changchun;Fang, Mingdi;Zhu, Mengjin;Li, Xinyun;Zhao, Shuhong;Chen, Hongbo;Li, Changchun;Fang, Mingdi;Zhu, Mengjin;Li, Xinyun;Zhao, Shuhong;Zhou, Rui;Li, Kui

作者机构：

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2009 年 10 卷

页码：

收录情况： SCI

摘要： Background: Haemophilus parasuis (HPS) is an important swine pathogen that causes Glasser's disease, which is characterized by fibrinous polyserositis, meningitis and arthritis. The molecular mechanisms that underlie the pathogenesis of the disease remain poorly understood, particularly the resistance of porcine immune system to HPS invasion. In this study, we investigated the global changes in gene expression in the spleen following HPS infection using the Affymetrix Porcine Genechip (TM). Results: A total of 931 differentially expressed (DE) transcripts were identified in the porcine spleen 7 days after HPS infection; of these, 92 unique genes showed differential expression patterns based on analysis using BLASTX and Gene Ontology. The DE genes involved in the immune response included genes for inflammasomes (RETN, S100A8, S100A9, S100A12), adhesion molecules (CLDN3, CSPG2, CD44, LGALS8), transcription factors (ZBTB16, SLC39A14, CEBPD, CEBPB), acute-phase proteins and complement (SAA1, LTF, HP, C3), differentiation genes for epithelial cells and keratinocytes (TGM1, MS4A8B, CSTA), and genes related to antigen processing and presentation (HLA-B, HLA-DRB1). Further immunostimulation analyses indicated that mRNA levels of S100A8, S100A9, and S100A12 in porcine PK-15 cells increased within 48 h and were sustained after administration of lipopolysaccharide (LPS) and Poly(I: C) respectively. In addition, mapping of DE genes to porcine health traits QTL regions showed that 70 genes were distributed in 7 different known porcine QTL regions. Finally, 10 DE genes were validated by quantitative PCR. Conclusion: Our findings demonstrate previously unrecognized changes in gene transcription that are associated with HPS infection in vivo, and many potential cascades identified in the study clearly merit further investigation. Our data provide new clues to the nature of the immune response in mammals, and we have identified candidate genes that are related to resistance to HPS.

分类号：

  • 相关文献
作者其他论文 更多>>
  • EXPERT expands prime editing efficiency and range of large fragment edits

    作者：Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Han, Xiaosong;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Li, Kui;Zhang, Jifeng;Xu, Jie

    关键词：

  • Uncovering Molecular Mechanisms of Feed Efficiency in Pigs Through Multi-Omics Analysis of the Jejunum

    作者：Zhang, Saixian;Qi, Xiaolong;Li, Jingjin;Liu, Yuwen;Zhang, Saixian;Xiang, Yue;Jian, Yaobang;Zhao, Qiulin;Sun, Jiahui;Huang, Yi;Xu, Jing;Zheng, Zhuqing;Fu, Liangliang;Li, Xinyun;Zhang, Saixian;Xiang, Yue;Jian, Yaobang;Zhao, Qiulin;Sun, Jiahui;Huang, Yi;Xu, Jing;Zheng, Zhuqing;Fu, Liangliang;Li, Xinyun

    关键词：feed efficiency; pigs; jejunum; RNA-seq; ATAC-seq; lipid metabolism; immune response

  • Genome-Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells

    作者：Zhu, Xiusheng;Huang, Lei;Li, Guoli;Deng, Biao;Wang, Xiaoxiao;Yang, Hu;Zhang, Yuanyuan;Wen, Qiuhan;Wang, Chao;Zhang, Jingshu;Li, Kui;Liu, Yuwen;Deng, Biao;Deng, Biao;Zhao, Yunxiang

    关键词：cis-regulatory elements; high-throughput; iPSCs; silencer; Ss-STARR-seq

  • Transcriptome analysis reveals no obvious unintended effects in the spleen of CRISPR/Cas9-mediated CD163 and pAPN double-knockout pigs

    作者：Liu, Zhi-Guo;Wang, Nan;Wang, Wan-Jie;Chi, Shun-Shun;Nie, Yu-Xin;Yuan, Mao-Sha;Sun, Ya-Ru;Mu, Yu-Lian;Li, Kui;Wang, Nan;Huang, Lei;Chi, Shun-Shun;Li, Kui

    关键词：CD163; pAPN; Double-knockout; Gene editing; Pig; Spleen; Unintended effects; Transcriptomics

  • Integrating large-scale meta-GWAS and PigGTEx resources to decipher the genetic basis of 232 complex traits in pigs

    作者：Xu, Zhiting;Lin, Qing;Cai, Xiaodian;Zhong, Zhanming;Teng, Jinyan;Zeng, Haonan;Gao, Yahui;Wei, Chen;Zhou, Jun;Zhang, Wenjing;Zhang, Xiaoke;Shi, Shaolei;Wu, Jun;Diao, Shuqi;Liu, Yuqiang;Pan, Xiangchun;Feng, Xueyan;Liu, Ruiqi;Su, Zhanqin;Chang, Chengjie;Zhu, Qianghui;Wu, Yuwei;Zhang, Hao;Yuan, Xiaolong;Li, Jiaqi;Zhang, Zhe;Li, Bingjie;Gao, Yahui;Liu, George E.;Gao, Yahui;Ma, Li;Cai, Zexi;Bai, Zhonghao;Su, Guosheng;Sahana, Goutam;Lund, Mogens Sando;Fang, Lingzhao;Wang, Xiaoqing;Shi, Liangyu;Zhao, Fuping;Wang, Xue;Wang, Yi;Zhang, Zipeng;Ding, Xiangdong;Lin, Yu;Tang, Guoqing;Li, Mingzhou;Li, Xuewei;Liu, Shuli;Yin, Hongwei;Bai, Lijing;Li, Kui;Zhou, Zhongyin;Wang, Qishan;Pan, Yuchun;Xu, Zhong;Peng, Xianwen;Mei, Shuqi;Mo, Delin;Liu, Xiaohong;Chen, Yaosheng;Liu, Yang;Xiang, Ruidong;Xiang, Ruidong;Shen, Xia;Shen, Xia;Shen, Xia;Li, Pinghua;Huang, Ruihua;Li, Pinghua;Huang, Ruihua;Ballester, Maria;Crespo-Piazuelo, Daniel;Amills, Marcel;Clop, Alex;Amills, Marcel;Karlskov-Mortensen, Peter;Fredholm, Merete;Zhang, Qin;Zhao, Yunxiang

    关键词：pig; meta-GWAS; PigGTEx; molQTL; complex traits

  • Identification of SepF in Streptococcus suis involving cell division

    作者：Gao, Ting;Zhu, Jiajia;Liu, Wei;Yang, Keli;Zhang, Tengfei;Yuan, Fangyan;Liu, Zewen;Guo, Rui;Li, Chang;Wu, Qiong;Tian, Yongxiang;Zhou, Danna;Li, Tingting;Zheng, Linlin;Zhou, Rui;Zheng, Linlin;Chen, Mo

    关键词：Streptococcus suis; Cell division; Septum; Protein-protein interaction; Drug target

  • Multi-dimensional annotation of porcine variants using genomic and epigenomic features in pigs

    作者：Yin, Hongwei;Yang, Liu;Zhao, Qianyi;Yao, Wenye;Bai, Lijing;Li, Kui;Fang, Lingzhao;Yao, Wenye;Groenen, Martien A. M.;Madsen, Ole;Teng, Jinyan;Gao, Yahui;Xu, Zhiting;Lin, Qing;Diao, Shuqi;Li, Jiaqi;Zhang, Zhe;Zhou, Huaijun;Guan, Dailu;Liu, Xiaohong;Zhao, Fuping;Zhou, Zhongyin;Wang, Qishan

    关键词：Genomic variants; Gene expression and splicing; Complex traits; Non-coding regions; Epigenome

微信小程序