Multiplex PCR-capillary electrophoresis-SSCP used to identify foodborne pathogens

文献类型： 外文期刊

第一作者： Wu, Yajun

作者： Wu, Yajun;Zhu, Changxiong;Wu, Yajun;Chen, Ying;Wang, Bin;Yang, Hairong;Yuan, Fei;Xu, Baoliang

作者机构：

关键词： Multiplex PCR-CE-SSCP;Bacteria;16s rRNA;GyrB

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：2.998； 五年影响因子：3.005 ）

ISSN： 1438-2377

年卷期： 2009 年 228 卷 4 期

页码：

收录情况： SCI

摘要： Multiplex PCR-CE-SSCP approach was used to identify foodborne pathogens. 16srRNA system including three target fragments localized at different position of 16srRNA gene and gyrB system including two target fragments at gyrB gene were established. SSCP profile was analyzed in a similar way as RAPD and the identity of each peak was determined by relative position to inner standard. Twenty-five reference bacteria strains representing 2 classes, 12 genus and 19 species were tested. All bacteria could be distinctly determined at genus level by 16srRNA system. GyrB system improved discriminating resolution of some bacterial at species level. Phylogenetic analysis showed that 16srRNA based phylogenetic relationship was consistent with traditional classification of these organisms. Good reproducibility and resolution make multiplex PCR-CE-SSCP protocol a promising approach for fast screening of foodborne pathogens.

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