PRODUCTION OF TRANSGENIC CHICKENS CARRYING THANATIN GENE BY TRANSFECTION OF SPERMATOGONIAL STEM CELLS

文献类型： 外文期刊

第一作者： Miao Xiangyang

作者： Miao Xiangyang;Qu Zhaojie;Zhu Ruiliang;Zhang Xin

作者机构：

关键词： Antimicrobial peptide thanatin;intratesticular injectio;transgenic chicken;expression

期刊名称：IFPT'6: PROGRESS ON POST-GENOME TECHNOLOGIES, PROCEEDINGS

ISSN：

年卷期： 2009 年

页码：

收录情况： SCI

摘要： Transgenic animal have extensive perspective of research and application in field of medicine, life science and biopharmacy. For this reason, scientists have been explored a more convenient and easy, economic and practical, and high-performance transgenic methods. Intratesticular injection as a method to transfer foreign gene into sperm can avoid inhibitory factor and complicated dealing of sperm, ensuring fertilization and embryo development. Recombinant efficient expression was made from pIRES2-EGFP plasmid backbone by inserting antimicrobial peptide -thanatin sequence constructed into the MCS region. Then the linear vector was injected into the testis of the BaiLai-hang chicken by surgical operation. 268 s were collected, 257 of them were transgenic chicken embryos. PCR and Southern blotting results revealed that 160 of them were positive transgenic chicken embryos, and transgenic rate was 59.70%. Southern blotting results showed that the foreign gene was integrated in chicken chromosomes with multi-inserted repeat sequences.

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