Isolation and Characterization of a Field Strain of Bovine Ephemeral Fever Virus in China

文献类型： 外文期刊

第一作者： Zheng, Fuying

作者： Zheng, Fuying;Lin, Guozhen;Qiu, Changqing;Zhou, Jizhang;Cao, Xiaoan;Gong, Xiaowei

作者机构：

关键词： BEFV;characterization;field strain;isolation;TEM;China

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2009 年 8 卷 8 期

页码：

收录情况： SCI

摘要： The infected blood was collected from dairy cattle showing similar Bovine Ephemera Fever (BEF) clinical signs in Taizhou area of Zhejiang province. The blood frozen-thawed 3 times was concentrated decuple by centrifugation, then the virus had 7 blind passages in suckling mice by an intracerebral inoculation route and 10 passages in BHK21 cells. The suckling mice died regularly 3-4 days after inoculation from the 3rd passage with 100% mortality rate. In BHK21 cells, the CPE occurred from the secondary passage and 80% cells approximately emerged pathological changes post-infection from the 4th passage. The virus isolated was proved to be Bovine Ephemera Fever Virus (BEFV) by RT-PCR, Transmission Electron Microscopy (TEM), Virus Neutralization (VN) test and IFA test. The virus isolated was designated JT02L. The homologies of amino acid sequence of the G gene compared with JB76H (Beijing strain), Taiwan strain and BB7721 (Australia's strain) all were above 90%.

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