Detecting a deletion in the coding region of the bovine bone morphogenetic protein 15 gene (BMP15)

文献类型： 外文期刊

第一作者： Xu, S-Z.

作者： Xu, S-Z.;Gan, Q-F.;Zhang, X-H.

作者机构：

关键词： bovine;bone morphogenetic protein 15 gene;deletion mutation

期刊名称：JOURNAL OF APPLIED GENETICS （ 影响因子：3.24； 五年影响因子：2.756 ）

ISSN： 1234-1983

年卷期： 2009 年 50 卷 2 期

页码：

收录情况： SCI

摘要： The aim of this study was to detect polymorphism in the bovine bone morphogenetic protein 15 (BMP15) gene. On the basis of PCR-SSCP and DNA sequencing, a 4-bp deletion was identified in the coding region of the gene. Sequence analysis revealed that the deletion altered the reading frame and introduced a stop codon at position 264. Eight breeds (Luxi, Qinchuan, Nanyang, Jinnan, Bohai Black, Menggolian, Holstein, and Simmental) were genotyped by PCR-SSCP. No cows homozygous for this Mutation were observed in these breeds. Heterozygous cows were detected in Luxi, Qinchuan, Nanyang, Jinnan and Bohai Black cattle. Fecundity was not increased in heterozygous individuals.

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