Integrated microfluidic immunoassay for the rapid determination of clenbuterol

文献类型： 外文期刊

第一作者： Kong, Jing

作者： Kong, Jing;Jiang, Lei;Qin, Jianhua;Du, Yuguang;Lin, Bingcheng;Kong, Jing;Su, Xiaoou

作者机构：

期刊名称：LAB ON A CHIP （ 影响因子：6.799； 五年影响因子：7.379 ）

ISSN： 1473-0197

年卷期： 2009 年 9 卷 11 期

页码：

收录情况： SCI

摘要： An integrated microfluidic immunoassay system was established for high throughput analysis of clenbuterol. This system consisted of an integrated microchip and a linear confocal laser induced fluorescence (LIF) scanner. The microchip was composed of three layers: a fluidic channel layer, a PDMS membrane layer and a pneumatic control layer. The multi-layer chip was integrated with 36 pneumatic micro-valves and multiple micro-pumps to realize the flexible reagent delivery, facilitating the automatic assays with less consumption of samples and reduced analysis time. The homemade LIF scanner was able to simultaneously detect multi-channels and provide the potential capability of high throughput assays. The performance of the system was demonstrated by the determination of clenbuterol, one of the most widely used beta-agonists. Under the optimal conditions, the linear range and the limit of detection of clenbuterol were 0 similar to 5.0 ng mL(-1) and 0.088 ng mL(-1), respectively. The recovery rates determined with pig urine samples of 1.0 ng mL(-1) and 2.0 ng mL(-1) were 98.74% and 102.51% (n = 3), respectively. The total detection time was less than 30 min. The system had the potential application for rapid detection of multiple beta-agonists in clinical, pharmaceutical and chemical analyses.

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