Development of a sequence-specific PCR marker linked to the gene "pauper'' conferring low-alkaloids in white lupin (Lupinus albus L.) for marker assisted selection

文献类型： 外文期刊

第一作者： Clements, Jonathon

作者： Clements, Jonathon;Adhikari, Kedar;Buirchell, Bevan;Sweetingham, Mark;Yang, Huaan;Lin, Ruiming;Luckett, David;Clements, Jonathon;Yan, Guijun

作者机构：

关键词： Marker assisted selection (MAS);Molecular marker;MFLP;Lupinus albus

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2009 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Seeds and plants of wild type Lupinus albus are bitter and contain high level of alkaloids. During domestication, at least three genes conferring low-alkaloid content were identified and incorporated into commercial varieties. Australian lupin breeders exclusively utilize one of these sweetness genes, "pauper'', in all varieties to prevent possible bitterness contamination via out-crossing. A cross was made between a sweet variety Kiev Mutant ( containing pauper gene) and a bitter type landrace P27174, and the population was advanced into F(8) recombinant inbred lines (RILs). Twenty-four plants representing sweetness and bitterness were subjected to DNA fingerprinting by the microsatellite-anchored fragment length polymorphism (MFLP) technique. A dominant polymorphism was discovered in an MFLP fingerprint. The MFLP marker was converted into a co-dominant, sequence-specific, simple PCR-based marker. Linkage analysis by the software program MapManager with marker score data and alkaloid phenotyping data from a segregating population containing 190 F(8) RILs indicated that the marker is linked to the pauper gene at the genetic distance of 1.4 centi-Morgans (cM). This marker, which is designated as "PauperM1'', is capable of distinguishing the pauper gene from the other two low-alkaloid genes exiguus and nutricius. Validation on germplasm from the Australian lupin breeding program showed that the banding pattern of the marker PauperM1 is consistent with the alkaloid genotyping on a wide range of domesticated varieties and breeding lines. The PauperM1 marker is now being implemented for marker assisted selection in the Australian albus lupin breeding program.

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