The expression of classical swine fever virus structural protein E2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors

文献类型: 外文期刊

第一作者: Shao, Hong-Bo

作者: Shao, Hong-Bo;Shao, Hong-Bo;Shao, Hong-Bo;Shao, Hong-Bo;Su, Zhong-Liang;He, Dong-Mei;He, Dong-Mei;Qian, Kai-Xian;Shen, Gui-Fang

作者机构:

关键词: classical swine fever virus (CSFV);E2 gene;chloroplast;immunogenicity;bioreactor;anti-disease plant-gene engineering

期刊名称:COMPTES RENDUS BIOLOGIES ( 影响因子:1.583; 五年影响因子:2.123 )

ISSN: 1631-0691

年卷期: 2008 年 331 卷 3 期

页码:

收录情况: SCI

摘要: It has been reported that genes encoding antigens of bacterial and viral pathogens can be expressed in plants and are shown to induce protection antibodies. The structural protein E2 of classical swine fever virus (CSFV), which has been shown to carry critical epitopes, has been expressed in different systems. Here, we report the expression of CFSV E2 gene in tobacco chloroplasts. Mice immunized with leaf extracts elicited specific antibodies. This indicated that the expressed E2 proteins had a certain degree of immunogenicity. To our knowledge, this is the first report showing induction of protective antibody in response to classical swine fever virus (CSFV) by immunization with antigen protein E2 expressed in tobacco chloroplasts, which will open a new way to protection from, CSFV by plant chloroplasts as bioreactors.

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