Effective production and purification of the glycosylated TSOL18 antigen, which is protective against pig cysticercosis

文献类型： 外文期刊

第一作者： Cai, Xuepeng

作者： Cai, Xuepeng;Yuan, Gailing;Zheng, Yadong;Luo, Xuenong;Zhang, Shaohua;Ding, Juntao;Jing, Zhizhong;Lu, Chengping

作者机构：

期刊名称：INFECTION AND IMMUNITY （ 影响因子：3.441； 五年影响因子：3.702 ）

ISSN： 0019-9567

年卷期： 2008 年 76 卷 2 期

页码：

收录情况： SCI

摘要： Cysticercosis caused by Taenia solium metacestodes is a worldwide public health problem. Important progress in the development of effective and practical vaccines against this disease has been made. In this study, the promising T. solium oncospheral vaccine candidate named TSOL18 antigen was produced in a 5-liter fermentor. During the process of fermentation, the pH of the culture was always kept below 5.0, and in order to prevent foaming, an antifoam agent was added. In addition, the oxygen content of the culture was constantly kept at >50% in our experiment. A high level of the glycosylated protein (2.5 g/liter) was obtained, and the protein was easily purified by gel chromatography. Vaccination trials showed that the recombinant TSOL18 antigen induced 94 and 100% reductions in metacestode burdens in vaccinated pigs, obviously higher than the 89% reduction in pigs immunized with cysticercus crude extracts in trial 1. These are very promising results in the development of an efficient tool to control cysticercosis in Asia.

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