A highly sensitive and specific multiplex RT-PCR to detect foot-and-mouth disease virus in tissue and food samples
文献类型： 外文期刊
第一作者： Bao, H. -F.
作者： Bao, H. -F.;Li, D.;Guo, J. -H.;Lu, Z. -J.;Chen, Y. -L.;Liu, Z. -X.;Liu, X. -T.;Xie, Q. -G.
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2008 年 153 卷 1 期
页码：
收录情况： SCI
摘要： Three sets of primers to detect foot-and-mouth disease virus (FMDV) using multiplex RT-PCR were designed based on several reference nucleotide sequences, and their reaction conditions were determined. By testing ten-fold serial dilutions of FMDV, the sensitivity of multiplex RT-PCR is 100 times higher than conventional RT-PCR. Meanwhile, its specificity was confirmed compared with other related vesicular disease viruses. Furthermore, 30 field samples from different animals were tested, and the results supported the method's potential applications in routine veterinary quarantine and epidemic surveillance of FMDV.
分类号：
- 相关文献
作者其他论文 更多>>
-
EFFECTS OF SOIL SALT CONCENTRATION ON nirS AND nosZ GENES DIVERSITY OF PADDY SOIL DENITRIFYING BACTERIA
作者：Ding, Y.;He, J.;Li, D.;Liu, G.;Liao, J.;Ma, Y.;Zhou, H.;Wu, Q.
关键词：Denitrifying bacteria; Functional denitrification gene; Salt concentration; Diversity; Soil
-
Identification and fine mapping of a new bacterial blight resistance gene, Xa43(t), in Zhangpu wild rice (Oryza rufipogon)
作者：Huang, F.;He, N.;Yu, M.;Yang, D.;Li, D.
关键词：Bacterial blight (BB) resistance; chromosome segment substitution line (CSSL); fine mapping; Rice; ZhangPu wild rice
-
Genome- Wide Association Study of Abdominal Fat in Wenshang Barred Chicken Based on the Slaf- Seq Technology
作者：Liu, W.;Liu, J.;Zhou, Y.;Cao, D.;Lei, Q.;Han, H.;Wang, J.;Li, D.;Gao, J.;Li, H.;Li, F.;Liu, W.;Liu, J.;Zhou, Y.;Cao, D.;Lei, Q.;Han, H.;Wang, J.;Li, D.;Gao, J.;Li, F.
关键词：Abdominal fat traits; genome-wide association study; SLAF-seq; Wenshang Barred chicken
-
Selection and implementation of single nucleotide polymorphism markers for parentage analysis in crossbred cattle population
作者：Hu, L. R.;Li, D.;Wang, Y. C.;Zhou, L.;Yu, Y.;Zhang, Y.;Zhang, S. L.;Usman, T.;Li, D.;Chu, Q.;Usman, T.;Xie, Z. Q.;Hou, S. Y.;Liu, L.;Shi, W. H.
关键词：Holstein cattle; On-farm records; Parental errors; Simmental cattle; Single nucleotide polymorphism assays
-
Effects of laying breeder hens dietary beta-carotene, curcumin, allicin, and sodium butyrate supplementation on the jejunal microbiota and immune response of their offspring chicks
作者：Gong, H. Z.;Lang, W. Y.;Lan, H. N.;Wang, T. P.;Chu, Q. R.;Zheng, X.;Wu, M.;Wang, J. H.;Li, D.;Zheng, X.;Fan, Y. Y.
关键词：laying breeder hen; beta-carotene; antibiotic; microbiota; immune response
-
EVALUATION OF HEAT TOLERANCE INDEX, SUSCEPTIBILITY INDEX OF CANOPY TEMPERATURE AND LEAF CHLOROPHYLL CONTENT OF WHEAT (TRITICUM AESTIVUM L.)
作者：Li, Q.;Meng, X. H.;Li, D.;Zhao, M. H.;Sun, S. L.;Li, H. M.;Li, W.;Qiao, W. C.;Li, Q.;Meng, X. H.;Li, D.;Zhao, M. H.;Sun, S. L.;Li, H. M.;Li, W.;Qiao, W. C.;Li, Q.;Meng, X. H.;Li, D.;Zhao, M. H.;Sun, S. L.;Li, H. M.;Li, W.;Qiao, W. C.
关键词：wheat; heat stress; stress intensity; yield; yield reduction
-
Different responses of soil organic carbon fractions to additions of nitrogen
作者：Chen, H.;Li, D.;Wang, K.;Chen, H.;Li, D.;Wang, K.;Feng, W.;Niu, S.;Plante, A. F.;Luo, Y.
关键词：
