Cloning and characterization of microRNAs from Brassica napus

文献类型： 外文期刊

第一作者： Wang, Lei

作者： Wang, Lei;Wang, Ming-Bo;Tu, Jin-Xing;Helliwell, Christopher A.;Waterhouse, Peter M.;Dennis, Elizabeth S.;Fu, Ting-Dong;Fan, Yun-Liu

作者机构：

关键词： heterosis;small RNAs;microRNAs;secondary structure;miR169;Brassica napus

期刊名称：FEBS LETTERS （ 影响因子：4.124； 五年影响因子：3.814 ）

ISSN： 0014-5793

年卷期： 2007 年 581 卷 20 期

页码：

收录情况： SCI

摘要： A library containing approximately 40,000 small RNA sequences was constructed for Brassica napus. Analysis of 3025 sequences obtained from this library resulted in the identification of 11 conserved miRNA families, which were validated by secondary structure prediction using surrounding sequences in the Brassica genome. Two 21 nt small RNA sequences reside within the arm of a pre-miRNA like stem-loop structure, making them likely candidates for novel non-conserved miRNAs in B. napus. Most of the conserved miRNAs were expressed at similar levels in a F1 hybrid A napus line and its four double haploid progeny that showed marked variations in phenotypes, but many were differentially expressed between B. napus and Arabidopsis. The miR169 family was expressed at high levels in young leaves and stems, but was undetectable in roots and mature leaves, suggesting that miR169 expression is developmentally regulated in B. napus. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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