Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.)

文献类型: 外文期刊

第一作者: Kong, W. D.

作者: Kong, W. D.;Zhu, Y. G.;Liang, Y. C.;Zhang, J.;Smith, F. A.;Yang, A.

作者机构:

关键词: alfalfa;Medicago sativa L.;oxytetracycline;uptake;veterinary pharmaceuticals

期刊名称:ENVIRONMENTAL POLLUTION ( 影响因子:8.071; 五年影响因子:8.35 )

ISSN: 0269-7491

年卷期: 2007 年 147 卷 1 期

页码:

收录情况: SCI

摘要: A series of experiments were conducted in a hydroponic system to investigate the uptake of oxytetracycline (OTC) and its toxicity to alfalfa (Medicago sativa L.). OTC inhibited alfalfa shoot and root growth by up to 61% and 85%, respectively. The kinetics of OTC uptake could be well described by Michaelis-Menten equation with V-max of 2.25 mu mol g(-1) fresh weight h(-1), and K-m of 0.036 mM. The uptake of OTC by alfalfa was strongly inhibited by the metabolic inhibitor, 2,4-DNP (2,4-dinitrophenol), at pH 3.5 and 6.0, but not by the aquaporin competitors, glycerol and Ag+. OTC uptake, however, was significantly inhibited by Hg2+, suggesting that the inhibition of influx was due to general cellular stress rather than the specific action of Hg2+ on aquaporins. Results from the present study suggested that OTC uptake into alfalfa is an energy-dependent process. (c) 2006 Elsevier Ltd. All rights reserved.

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