A 38-kDa protein from Babesia gibsoni and its antibody response in an experimentally infected dog

文献类型： 外文期刊

第一作者： Zhou, Jinlin

作者： Zhou, Jinlin;Zhang, Guohong;Nishikawa, Yoshifumi;Fujisaki, Kozo;Xuan, Xuenan

作者机构：

关键词： Babesia gibsoni;12D3 antigen;antibody response

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN： 0304-4017

年卷期： 2006 年 141 卷 3-4 期

页码：

收录情况： SCI

摘要： A cDNA encoding the Babesia bovis 12D3 antigen homologue was obtained by immunoscreening the expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1406 bp. Computer analysis suggested that the sequence contains an open reading frame of 1052 bp encoding an expected protein with a molecular weight of 36 kDa. Based on homology analysis, this putative protein was designated as the B. gibsoni 12133 antigen (Bg12D3). The Bg12D3 gene was expressed in the Escherichia coli BL21 strain, and the chronically infected dog serum reacted with the recombinant protein. The antiserum against the recombinant Bg12D3 protein can recognize a 3 8-kDa native protein, which is consistent with its expected size. Moreover, the purified recombinant proteins were used as the antigen to detect the antibody response in an experimentally infected dog by the enzyme-linked immunosorbent assay (ELISA). Our results indicated that the Bg12D3 protein was recognized by the host immune system and that it induced an antibody response in chronic B. gibsoni infection. These results allowed us to identify a new member of the 12D3 antigens and its characteristic immune response in canine B. gibsoni infection. (c) 2006 Elsevier B.V. All rights reserved.

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