Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco
文献类型: 外文期刊
第一作者: Gu, Riliang
作者: Gu, Riliang;Zhao, Li;Zhang, Ying;Chen, Xiaoping;Bao, Juan;Zhao, Jinfeng;Wang, Zhangying;Fu, Junjie;Liu, Tingsong;Wang, Jianhua;Wang, Guoying
作者机构:
关键词: beta-glucosidase;cytokinin;expression pattern;GUS;promoter
期刊名称:PLANT CELL REPORTS ( 影响因子:4.57; 五年影响因子:4.463 )
ISSN: 0721-7714
年卷期: 2006 年 25 卷 11 期
页码:
收录情况: SCI
摘要: The beta-glucosidase gene of maize (ZmGLU1) was suggested to hydrolyze cytokinin-conjugate and release free cytokinin during plant growth and development. A clone containing the upstream region of ZmGLU1 was isolated and sequenced from a maize genomic library. The full-length ZmGLU1 promoter and a series of its 5' deletions were fused to the beta-glucuronidase (GUS) reporter gene and transferred into tobacco. The GUS activity of transgenic plants was assayed at various developmental stages. The results showed that ZmGLU1 promoter-driven GUS gene had the highest expression level in the roots and that the expression of GUS gene declined during seed maturation and down to the lowest level in mature seeds. The ZmGLU1 promoter-driven GUS expression increased during seed germination, reaching a peak on day 11. The results also showed that this promoter could be inhibited by 6-BA, trans-zeatin, and NAA, but was not affected by GA(3), ABA, SA, cold, salt, drought, and submergence treatments. The histochemical staining revealed that GUS activity was located in vigorous cell division zones with dominant staining associated with vascular tissues. Deletion analysis showed that the promoter contained a putative leaf-specific and stem-specific negative regulative element and two putative enhancers.
分类号:
- 相关文献
作者其他论文 更多>>
-
Dynamic changes of quality characteristics during fermentation of orange-fleshed sweet potato alcoholic beverage
作者:Wu, Huimin;Fu, Qinquan;Tang, Tiantian;Liu, Yinqi;Ma, Xinyi;Zhao, Min;Zhou, Xin;Wang, Bin;Shi, Xuewei;Yang, Yiling;Wang, Zhangying;Yang, Yiling;Wang, Zhangying;Wang, Bin;Shi, Xuewei;Wang, Bin;Shi, Xuewei;Wang, Bin;Shi, Xuewei
关键词:Orange-fleshed sweet potato (OFSP); Alcoholic beverage; Antioxidant properties; Physicochemical indicators; Volatile compounds
-
Bioenhanced remediation of dibutyl phthalate contaminated black soil by immobilized biochar microbiota
作者:Tao, Yue;Wang, Yao;Cui, Yunhe;Sun, Rui;Zhang, Bo;Qu, Jianhua;Zhang, Ying;Cai, Hongguang
关键词:Biochar; DBP; Microbial remediation; Black soil; Soil nutrient
-
Simultaneous profiling of chromatin-associated RNA at targeted DNA loci and RNA-RNA Interactions through TaDRIM-seq
作者:Ding, Cheng;Chen, Guoting;Luan, Shiping;Gao, Runxin;Fan, Yudong;Zhang, Ying;Wang, Xiaoting;Li, Guoliang;Foda, Mohamed F.;Yan, Jiapei;Li, Xingwang;Chen, Guoting;Li, Guoliang;Chen, Guoting;Li, Guoliang;Foda, Mohamed F.;Li, Xingwang;Li, Xingwang
关键词:
-
Enhanced removal of tetracycline from water using MgO-modified g-C3N4 composite: Synthesis optimization and mechanism investigation
作者:Yu, Hui;Gao, Longfei;Zhang, Xinyuan;Zhang, Shuang;Chi, Wenshi;Zhang, Long;Li, Jianzhuo;Tian, Yushi;Zhang, Ying;Cai, Hongguang
关键词:Heterojunction; Photocatalysis; Tetracycline degradation; Wastewater treatment
-
Pan-genome bridges wheat structural variations with habitat and breeding
作者:Jiao, Chengzhi;Hao, Chenyang;Xie, Yuxin;Zhao, Li;Li, Tian;Fu, Junjie;Hou, Jian;Liu, Hongxia;Liu, Xu;Jia, Jizeng;Mao, Long;Zhang, Xueyong;Jiao, Chengzhi;Wang, Xiue;Xie, Xiaoming;Wang, Zihao;Zhang, Yuqi;Guo, Weilong;Chen, Liyang;Garg, Vanika;Chitikineni, Annapurna;Appels, Rudi;Varshney, Rajeev K.;Dwivedi, Girish;Dwivedi, Girish;Appels, Rudi
关键词:
-
Enhanced remediation of cadmium-contaminated farmland by smooth vetch (Vicia villosa var.) coupled with phosphorus/sulfur co-doped biochar: Synergetic performance and mechanism
作者:Bi, Fuxuan;Jiang, Zhao;Wang, Mengning;Lin, Qiutong;Liu, Xiaoyang;Qu, Jianhua;Zhang, Ying;Fu, Libo;Fan, Hongli;Cao, Weidong
关键词:P/S co-doped; Biochar; Smooth vetch; Cadmium; Synergistic remediation
-
Enrichment of carcinoembryonic antigen by epitope molecularly imprinted polymer based on cucurbituril host-guest interaction
作者:Xu, Yitong;Zhang, Ying;He, Yi;Jia, Qiong;Zhang, Huifeng
关键词:Epitope imprinting; Cucurbit[n]uril; Host-guest interaction; Carcinoembryonic antigen