Isolation of a maize beta-glucosidase gene promoter and characterization of its activity in transgenic tobacco
文献类型: 外文期刊
第一作者: Gu, Riliang
作者: Gu, Riliang;Zhao, Li;Zhang, Ying;Chen, Xiaoping;Bao, Juan;Zhao, Jinfeng;Wang, Zhangying;Fu, Junjie;Liu, Tingsong;Wang, Jianhua;Wang, Guoying
作者机构:
关键词: beta-glucosidase;cytokinin;expression pattern;GUS;promoter
期刊名称:PLANT CELL REPORTS ( 影响因子:4.57; 五年影响因子:4.463 )
ISSN: 0721-7714
年卷期: 2006 年 25 卷 11 期
页码:
收录情况: SCI
摘要: The beta-glucosidase gene of maize (ZmGLU1) was suggested to hydrolyze cytokinin-conjugate and release free cytokinin during plant growth and development. A clone containing the upstream region of ZmGLU1 was isolated and sequenced from a maize genomic library. The full-length ZmGLU1 promoter and a series of its 5' deletions were fused to the beta-glucuronidase (GUS) reporter gene and transferred into tobacco. The GUS activity of transgenic plants was assayed at various developmental stages. The results showed that ZmGLU1 promoter-driven GUS gene had the highest expression level in the roots and that the expression of GUS gene declined during seed maturation and down to the lowest level in mature seeds. The ZmGLU1 promoter-driven GUS expression increased during seed germination, reaching a peak on day 11. The results also showed that this promoter could be inhibited by 6-BA, trans-zeatin, and NAA, but was not affected by GA(3), ABA, SA, cold, salt, drought, and submergence treatments. The histochemical staining revealed that GUS activity was located in vigorous cell division zones with dominant staining associated with vascular tissues. Deletion analysis showed that the promoter contained a putative leaf-specific and stem-specific negative regulative element and two putative enhancers.
分类号:
- 相关文献
作者其他论文 更多>>
-
Insights into the effects of anilofos on direct-seeded rice production system through untargeted metabolomics
作者:Wang, Weitao;Wang, Weitao;Long, Jiahuan;Huang, Wenyuan;Zhang, Ying;Duan, Tingting;Wang, Huaixu
关键词:Direct-seeded rice; Anilofos; Untargeted metabolomics; KEGG pathway analysis
-
Integrated Transcriptomic and Metabolomic Analysis Revealed Abscisic Acid-Induced Regulation of Monoterpene Biosynthesis in Grape Berries
作者:Li, Xiangyi;Yan, Yixuan;Wang, Lei;Li, Guanhan;Xu, Lurong;Wang, Shiping;Wu, Yusen;Zhang, Ying
关键词:abscisic acid (ABA); monoterpenes; transcriptome; HS-SPME/GC-MS; Ruiduhongyu; grape berries
-
Research on species identification of wild grape leaves based on deep learning
作者:Pan, Bowen;Liu, Chonghuai;Fan, Xiucai;Zhang, Ying;Sun, Lei;Jiang, Jianfu;Pan, Bowen;Ju, Yanlun;Fang, Yulin;Su, Baofeng;Jiang, Jianfu
关键词:Wild grapes; Deep learning; Species identification; Grad-CAM
-
Metabolic profiles and morphological characteristics of leaf tips among different sweet potato (Ipomoea batatas Lam.) varieties
作者:Tan, Wenqing;Wang, Zhangying;Zhang, Rong;Tang, Chaochen;Jiang, Bingzhi;Jia, Ruixue;Yang, Shaohai;Chen, Jingyi;Guo, Xinbo;Deng, Yuanyuan
关键词:sweet potato; leaf tips; phenotypic traits; metabolic profile; differentially expressed metabolites; polyphenols
-
New vegetable field converted from rice paddy increases net economic benefits at the expense of enhanced carbon and nitrogen footprints
作者:He, Zhilong;Xu, Minggang;Zhang, Wenju;Wu, Lei;He, Zhilong;Zhang, Ying;Hu, Ronggui;Wu, Xian;Tang, Shuirong;Zhang, Ying;He, Zhilong;Wu, Lei
关键词:Newly converted vegetable field; Life cycle assessment; Carbon and nitrogen footprints; Net ecosystem economic benefit
-
The cysteine-rich receptor-like kinase CRK10 targeted by Coniella diplodiella effector CdE1 contributes to white rot resistance in grapevine
作者:Liu, Ruitao;Tan, Xibei;Lin, Feng;Li, Peng;Rahman, Faiz Ur;Sun, Lei;Jiang, Jianfu;Fan, Xiucai;Liu, Chonghuai;Zhang, Ying;Liu, Ruitao;Zhang, Ying;Wang, Yiming;Lin, Feng
关键词:Effector; grapevine; Coniella diplodiella; cysteine-rich receptor-like kinase (CRK); grape white rot; disease resistance
-
Silica nanoparticles conferring resistance to bacterial wilt in peanut (Arachis hypogaea L.)
作者:Deng, Quanqing;Liu, Hao;Lu, Qing;Du, Puxuan;Li, Haifen;Li, Shaoxiong;Liu, Haiyan;Wang, Runfeng;Huang, Lu;Chen, Xiaoping;Hong, Yanbin;Huang, Suihua;Sun, Dayuan;Wu, Yahui
关键词:Peanut; Silica nanoparticles; Bacterial wilt resistance; Ralstonia solanacearum; Salicylic acid metabolism