Evaluation of transgenic rice expressing Cry1Ac and CpTI against Chilo suppressalis and intrapopulation variation in susceptibility to Cry1Ac

文献类型： 外文期刊

第一作者： Wu, Kong-Ming

作者： Wu, Kong-Ming;Peng, Yu-Fa;Wang, Feng;Guo, Yu-Yuan

作者机构：

关键词： transgenic rice lines;Chilo suppressalis;bioassay;variation in susceptibility;Cry1Ac

期刊名称：ENVIRONMENTAL ENTOMOLOGY （ 影响因子：2.377； 五年影响因子：2.298 ）

ISSN： 0046-225X

年卷期： 2006 年 35 卷 5 期

页码：

收录情况： SCI

摘要： The field efficacy of two transgenic rice lines, containing the Cry1Ac gene from Bacillus thuringiensis (Bt) and the CpTI (cowpea trypsin inhibitor) gene, on the striped stem borer, Chilo suppressalis (Walker), and population variation in susceptibility of C. suppressalis to the Bt insecticidal protein Cry1Ac were evaluated in Fuzhou, Fujian Province of China. The results of an open field test showed that the two transgenic lines exhibited high efficacy against natural infestations of C. suppressalis. In contrast, the untransformed parental control line showed serious damage symptoms not only in untreated plots, but also in plots treated with insecticides. The cumulative number of C. suppressalis adults derived from transgenic rice lines was significantly lower than that from control lines with and without chemical control treatments. Our results show that transgenic rice lines expressing Cry1Ac and CpTI have great potential for protecting rice from C. suppressalis damage with reduced use of insecticides. Comparison of pupal weight and fecundity of C. suppressalis between transgenic and control treatments indicated that pupal weight significantly decreased after continuously feeding on transgenic rice lines, but there was no significant effect on fecundity. Adult females of C. suppressalis were collected from light traps to ascertain population variation in susceptibility to Bt toxins. Each female producing enough fertile eggs for bioassays was used as the parent of an isofemale line. Of 1,100 females collected, 395 isofemale lines produced enough offspring to be screened on a diet assay treated with Cry1Ac. The results showed that 51.7% of isofemale lines were differentiated as highly susceptible, 23.0% as moderately susceptible, and 23.8% with low susceptibility; 1.5% were nonsusceptible, with a survival rate > 70% and similar development as on the non-Bt control diet. The results indicated significant variation in susceptibility to Cry1Ac in the C. suppressalis population, which should be considered for insect resistance management of Bt rice in the future.

分类号：

  • 相关文献

[1]Determination of baseline susceptibility to Cry1Ab protein for Asian corn borer (Lep., Crambidae). Wang, Z,Wen, L,Bai, S,Ma, X,Yao, Z. 2005

[2]Field-evolved resistance to insecticides in the invasive western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in China. Gong, Ya-Jun,Jin, Gui-Hua,Li, Bing-Yan,Chen, Jin-Cui,Kang, Zong-Jiang,Zhu, Liang,Wei, Shu-Jun,Gao, Yu-Lin,Reitz, Stuart.

[3]Morphological, phylogenetic and biological characteristics of Ectropis obliqua single-nucleocapsid nucleopolyhedrovirus. Ma, XC,Xu, HJ,Tang, MJ,Xiao, Q,Hong, J,Zhang, CX. 2006

[4]Establishment of a Sensitive Nitric Oxide Bioassay for Bioscreening Application. Yang, Qiao,Zhang, Xiaoling,Huang, Hongliang. 2014

[5]Correlation between pesticide resistance and enzyme activity in the diamondback moth, Plutella xylostella. Gong, Ya-Jun,Wang, Ze-Hua,Shi, Bao-Cai,Kang, Zong-Jiang,Zhu, Liang,Jin, Gui-Hua,Wei, Shu-Jun. 2013

[6]Screening of tropical isolates of Metarhizium anisopliae for virulence to the red palm weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae). Sun, Xiaodong,Yan, Wei,Qin, Weiquan,Zhang, Jing,Niu, Xiaoqing,Sun, Xiaodong,Li, Fuheng,Ma, Guangchang. 2016

[7]Biological Activity and Component Analysis on Body Surface Extracts of Adult Tessaratoma papillosa. Zhao, Dongxiang,Wang, Yujie,Gao, Jinglin,Peng, Zhengqiang,Yin, Jiong,Zhu, Wenjing. 2012

[8]Baseline susceptibility of Cnaphalocrocis medinalis (Lepidoptera : Pyralidae) to Bacillus thuringiensis toxins in China. Hou, M. L.,Peng, Y. F.,Liu, P. L..

[9]Allelopathic effects of aerial parts of Descurainia sophia L. on wheat. Liu, X. G.,Lu, C. H.,Dong, F. S.,Xu, J.,Wu, Y. B.,Zheng, Y. Q.,Tian, F. J.,Wu, Y. B.. 2016

[10]First record of Aspergillus oryzae (Eurotiales: Trichocomaceae) as an entomopathogenic fungus of the locust, Locusta migratoria (Orthoptera: Acrididae). You, Yinwei,Zhang, Long,You, Yinwei,Song, Yuan,Wang, Youzhi.

[11]Expression of Cry1Ab Protein in a Marker-Free Transgenic Bt Rice Line and Its Efficacy in Controlling a Target Pest, Chilo suppressalis (Lepidoptera: Crambidae). Li, Yunhe,Liu, Yanmin,Han, Lanzhi,Peng, Yufa,Zhang, Lei,Zhu, Zhen,Wang, Feng. 2014

[12]Field resistance to spinosad in western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae). Lei Zhong-ren,Gao Yu-lin,Li Dong-gang,Shang Xiao-yong,Reitz, Stuart,Nauen, Ralf,Si Hyeock Lee. 2016

[13]Identification and Field Evaluation of Non-Host Volatiles Disturbing Host Location by the Tea Geometrid, Ectropis obliqua. Zhang, Zheng-qun,Sun, Xiao-ling,Xin, Zhao-jun,Luo, Zong-xiu,Gao, Yu,Bian, Lei,Chen, Zong-mao.

[14]Acute toxicity of five pesticides to Apis mellifera larvae reared in vitro. Dai, Pingli,Dai, Pingli,Jack, Cameron J.,Mortensen, Ashley N.,Ellis, James D..

[15]Silencing of molt-regulating transcription factor gene, CiHR3, affects growth and development of sugarcane stem borer, Chilo infuscatellus. Zhang, Shu-zhen,Wu, Su-ran,Yu, Nai-tong,Wang, Jian-hua,Liu, Zhi-xin,Kulye, Mahesh,Zeng, Hong-mei. 2012

[16]Toxicity and affecting factors of Bacillus thuringiensis var. israelensis on Chironomus kiiensis larvae. Cao, Chuan-Wang,Sun, Li-Li,Wen, Rong-Rong,Li, Xiao-Peng,Wang, Zhi-Ying,Wu, Hong-Qu. 2012

[17]Biological Activity and Component Analysis on Body Surface Extracts of Adult Tessaratoma papillosa. Zhao, Dongxiang,Wang, Yujie,Gao, Jinglin,Peng, Zhengqiang,Yin, Jiong,Zhu, Wenjing. 2011

[18]Resistance monitoring of Chilo suppressalis (Walker) (Lepidoptera: Crambidae) to chlorantraniliprole in eight field populations from east and central China. Lu, Yanhui,Bai, Qi,Zheng, Xusong,Lu, Zhongxian,Wang, Guorong,Zhong, Liequan,Zhang, Facheng.

[19]An aptamer based assay for the beta-adrenergic agonist ractopamine based on aggregation of gold nanoparticles in combination with a molecularly imprinted polymer. Wang, Peilong,Su, Xiaoou,Shi, Lei,Yuan, Yuwei.

[20]Split aptamer-based sandwich fluorescence resonance energy transfer assay for 19-nortestosterone. Bai, Wenhui,Zhu, Chao,Liu, Jinchuan,Yan, Mengmeng,Yang, Shuming,Chen, Ailiang,Bai, Wenhui,Zhu, Chao,Liu, Jinchuan,Yan, Mengmeng,Yang, Shuming,Chen, Ailiang.

作者其他论文 更多>>

微信小程序