Study on the RNA interference of Sry in mouse embryos

文献类型： 外文期刊

第一作者： Zhao Jin-Hong

作者： Zhao Jin-Hong;Du Wei-Hua;Dao Er-Ji;Pei Jie;Zhang Li;Lin Xiu-Kun

作者机构：

关键词： siRNA;Sry;mouse embryos;gene silencing

期刊名称：PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS （ 影响因子：0.351； 五年影响因子：0.272 ）

ISSN： 1000-3282

年卷期： 2006 年 33 卷 9 期

页码：

收录情况： SCI

摘要： In order to study the regulation mechanism of Sry in sex determination, the siRNA technique was used to silence the. Sty expression in mouse embryos. Two siRNA expression vectors were synthesized, pSilencer4.1/Sry217 and pSilencer 4.1/Sry565. The siRNA expression vectors were injected into gestated mouse through tail vein. The sex was identified by PCR, and the expression level of Sry gene was determined using RT-PCR technique in the male mouse embryos at 11.5 dpc(days post coitum). The time- and dose -dependent effects was studied. The results confirmed that one of the siRNA expression plasmids, pSilencer4.1/Sry565, could inhibit the Sry gene expression significantly. Eighty-five percent inhibition was obtained when injecting 20 mu g siRNA vector at 9.5 dpc.

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