Characterization of the Babesia gibsoni P18 as a homologue of thrombospondin related adhesive protein

文献类型： 外文期刊

第一作者： Zhou, Jinlin

作者： Zhou, Jinlin;Fukumoto, Shinya;Jia, Honglin;Yokoyama, Naoaki;Zhang, Guohong;Fujisaki, Kozo;Lin, Jiaojiao;Xuan, Xuenan

作者机构：

关键词： Babesia gibsoni;TRAP;growth inhibition;SCID mice

期刊名称：MOLECULAR AND BIOCHEMICAL PARASITOLOGY （ 影响因子：1.759； 五年影响因子：2.135 ）

ISSN： 0166-6851

年卷期： 2006 年 148 卷 2 期

页码：

收录情况： SCI

摘要： Thrombospondin related adhesive proteins (TRAPs) are well conserved among several apicomplexans. In this study, we reported the identification of the Babesia gibsoni P18, designated by our group previously, as a homologue of TRAP and renamed the P18 as the B. gibsoni TRAP (BgTRAP). The amino acid sequence of BgTRAP consists of several typical regions, including a signal peptide, a vonWillebrand factor A domain, a thrombospondin type 1 domain, a transmembrane region, and a cytoplasmic C-terminus. The B. gibsoni infected dog serum recognized recombinant BgTRAP expressed in E. coli by Western blotting. The antiserum against recombinant BgTRAP recognized an 80 kDa protein in the lysate of infected erythrocytes (RBCs), which was detectable in the micronemal area of the parasites by confocal microscopic observation. The BgTRAP showed a bivalent cation-independent binding to canine RBC, and the specific antiserum was found to inhibit the growth of B. gibsoni in the infected severe combined immune deficiency mice given canine RBC. These results suggest that the BgTRAP is a new member of TRAP family identified from the merozoites of B. gibsoni and functionally important in merozoite invasion; this protein may be useful as a vaccine candidate against canine B. gibsoni infection. (c) 2006 Elsevier B.V. All rights reserved.

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