Genetic analysis of glyphosate tolerance in Halomonas variabilis strain HTG(7)

文献类型： 外文期刊

第一作者： Liu, Zhu

作者： Liu, Zhu;Lu, Wei;Chen, Ming;Yang, Zhirong;Lin, Min

作者机构：

关键词： EPSP synthase(5-enolpyruvylshikimate-3-phosphate synthase);glyphosate tolerance;Halomonas variabilis;sequence analysis

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN： 0959-3993

年卷期： 2006 年 22 卷 7 期

页码：

收录情况： SCI

摘要： A highly glyphosate-tolerant bacterium strain HTG(7) was isolated from glyphosate-polluted soil in north China, and identified as Halomonas variabilis. It was a Gram-negative motile rod giving convex colony. The strain HTG(7) could tolerate up to 900 mM glyphosate in minimal medium. The 16S rDNA sequence was amplified by PCR using universal primers. The region essential for glyphosate tolerance was localized to a 3.5-kb fragment from a cosmid library of HTG(7). The DNA fragment consisted of one complete open reading frame (ORF) and one partial ORF. The partial ORF was homologous to prephenate dehydrogenase of Pseudomonas aeruginosa PA01. The complete ORF contained the tyrA and aroA genes. Only the 1.35-kb aroA encoding EPSP synthase conferred glyphosate tolerance, and complemented with E. coli aroA mutant ER2799. E. coli JM109 harboring aroA grew well in Mops supplemented with 80 mM glyphosate.

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