Morphological, phylogenetic and biological characteristics of Ectropis obliqua single-nucleocapsid nucleopolyhedrovirus

文献类型: 外文期刊

第一作者: Ma, XC

作者: Ma, XC;Xu, HJ;Tang, MJ;Xiao, Q;Hong, J;Zhang, CX

作者机构:

关键词: Ectropis obliqua;single-nucleocapsid nucleopolyhedrovirus;polyhedrin;phylogeny;bioassay

期刊名称:JOURNAL OF MICROBIOLOGY ( 影响因子:3.422; 五年影响因子:3.28 )

ISSN: 1225-8873

年卷期: 2006 年 44 卷 1 期

页码:

收录情况: SCI

摘要: The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to 1.7 mu m in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rr1, polyhedrin, orf1629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration (LC50 and LC90) and lethal time (LT50 and LT90) were conducted to test the susceptibility of E. obliqua larvae to the virus.

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