Development and characterization of common wheat-Thinopyrum intermedium translocation lines with resistance to barley yellow dwarf virus

文献类型： 外文期刊

第一作者： Xin, ZY

作者： Xin, ZY;Zhang, ZY;Chen, X;Lin, ZS;Ma, YZ;Xu, HJ;Banks, PM;Larkin, PJ

作者机构：

关键词： BYDV;GISH;homoeologous group 7;RFLP;Thinopyrum intermedium;translocation

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2001 年 119 卷 1-2 期

页码：

收录情况： SCI

摘要： We developed some wheat-Th. intermedium translocation lines, Yw642, Yw443 and Yw243, etc., showing good BYDV resistance from L1 by induced homoeologous pairing using CS ph mutant. Characterization of these wheat lines was carried out by GISH and RFLP analysis. The results of GISH showed that the lines, YWw42, Yw443 and Yw243, etc., are homozygous wheat- Th. intermedium translocation lines, in which the chromosome segments of Th. intermedium were transferred to the distal end of a pair of wheat chromosomes. RFLP analysis indicated that the translocation chromosome of the wheat lines is T7DS.7DL-7XL. The breakpoint of the translocation is located on the distal end of 7DL, between Xpsr965 and Xpsr680 about 90-99 cm from the centromere. The BYDV gene is located on the distal end of 7XL around Xpsr680, Xpsr687 and Xwg380. The RFLP markers of psr680, psr687 and wg380 were cosegregated with the BYDV resistance respectively and could be used for molecular assisted selection (MAS) in wheat breeding program for BYDV resistance.

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