Identification and characterization of reverse transcriptase domain of mranscriptionally active retrotransposons in wheat genomes

文献类型： 外文期刊

第一作者： Tang, YM

作者： Tang, YM;Ma, YZ;Li, LC;Ye, XG

作者机构：

关键词： abiotic stress;biotic stress;retrotransposons;reverse transcriptase;Triticum;wheat

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2005 年 47 卷 5 期

页码：

收录情况： SCI

摘要： To clarify activation characterization of wheat (Triticum aestivum L.) retrotransposons, transcriptionally active Ty1-copia retrotransposons were found in wheat by using RT-PCR to amplify the RT domain. Sequence analysis of random RT-PCR clones reveals that Ty1-copia retrotransposons are highly heterogeneous and can be divided into at least four groups, which are tentatively named TaRT-1 to TaRT-4. Dot blot hybridization indicates that TaRT-1 exists in the wheat genome as multiple copies (at 30 000 copies/ a hexaploid genome (ABD)). Northern blot hybridization showed that TaRT- I is only expressed at a low level under normal conditions in seedlings, but at a high level when induced by powdery mildew fungus, jasmonic acid (JA) and salicylic acid (SA). These results suggest that the TaRT-1 expression is highly sensitive to biotic and abiotic stresses.

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