Characterization of a wheat-wheatgrass translocation line by fish

文献类型： 外文期刊

第一作者： Han, FP

作者： Han, FP;He, MY;Bu, XL;Huang, BQ;Hao, S;Ma, YZ;Xin, ZY

作者机构：

关键词： wheat;wheatgrass;translocation line;fluorescence in situ hybridization

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 1998 年 40 卷 6 期

页码：

收录情况： SCI

摘要： The genomic DNA of wheatgrass (Agropyron intermedium (Host) P. B. = Elytrigia intermedia (Host) Nevski = Thinopyrum intermedium (Host) Barkworth and Dewey) was labelled with biotin-16-dUTP as a probe, and genomic DNA of common wheat (Triticum aestivum L.) "Chinese Spring" was used for blocking. Wheat-wheatgrass line 33 was examined by fluorescence in situ hybridization (FISH) technique. The terminal regions of a pair of chromosomes showed green fluorescent signals. It has been concluded that chromosome segments containing alien genes of wheatgrass are located at the terminal regions of wheat chromosomes in wheat-wheatgrass line 33, and the translocated segments were small. Wheat-wheatgrass line 33 has been proved to be a translocation line with chromosome segments of wheatgrass translocated to the terminal regions of wheat chromosomes.

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