Karyotyping of Brassica napus l. Based on C(0)t-1 DNA banding by fluorescence In situ hybridization

文献类型： 外文期刊

第一作者： Wei, WH

作者： Wei, WH;Zhao, WP;Wang, LJ;Chen, B;Li, YC;Song, YC

作者机构：

关键词： Brassica napus;C(0)t-1 DNA;chromosome banding;fluorescence in situ hybridization (FISH);karyotyping

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2005 年 47 卷 12 期

页码：

收录情况： SCI

摘要： In order to precisely recognize and karyotype Brassica napus L. chromosomes, C(0)t-1 DNA was extracted from its genomic DNA, labeled withbiotin-11-dUTP and in situ hybridized. The hybridized locations were detected by Cy3-conjugated streptavidin. Specific fluorescence in situ hybridization (FISH) signal bands were detected on all individual chromosome pairs. Each chromosome pair showed specific banding patterns. The B. napus karyotype has been constructed, for the first time, on the basis of both C(0)t-1 DNA FISH banding patterns and chromosome morphology.

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