The sense and antisense expression of gibberellin 20-oxidase gene (rga5) in rice and its effects on GA(1) level and agronomic traits

文献类型： 外文期刊

第一作者： Yan, YX

作者： Yan, YX;An, CG;Li, L;Gu, JY;Wang, LJ;Mi, SL;Chen, ZL

作者机构：

关键词： Oryza sativa;gibberellin 20-oxidase;sense;antisense;transgenic

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2003 年 48 卷 5 期

页码：

收录情况： SCI

摘要： A gibberellin 20-oxidase gene rga5 was isolated by PCR from genomic DNA of rice (Oryza sativa ssp indica) cultivars 'Aizizhan' and 'Nante'. Compared with the reported OsGA20ox, the rga5 was partial-frame-shifted with 11 different amino acids. Then the rga5 with CaMV 35S promotor and NOS terminator was inserted into the poly-linker site of pCambia1301 to construct sense and antisense gene expressing vectors pSrga5 and pArga5. The transgenic plants were obtained by biolistic bombardment with pSrga5 or pArga5. The transgenic rice plants showed that the overexpression and antisense-expression of rga5 have remarkable effects on the biological characters of rice. The sense transgenic plants showed heightening with longer spike, more seed-bearing and unaffected flowering, whereas antisense transgenic plants showed dwarfing, early-flowering with slender stem, dark leaf color, shorter leaf and shorter spike. The PCR amplification and Southern blot hybridization showed that the rga5 has been integrated into the transgenic rice genome and the transcription of rga5 was identified by Northern blot hybridization. In the sense transgenic plants the GA(1) content increased of about 50%, however, the antisense transgenic rice decreased of 90% approximately compared with control plant 'Zhonghua 8'. These results demonstrated that the rga5 is a functional gene encoding gibberellin 20-oxidase in rice. Over-expressing rga5 significantly increases the endogenous GA(1) level and plant height of rice, whereas the expression of antisense rga5 decreases the GA(1) level and plant height of rice dramatically.

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