Entity evidence for differentiation between Tia and Tib types of soybean Kunitz trypsin inhibitor: detection of a novel transitional variant type between Tia and Tib in wild soybean (Glycine soja Sieb. & Zucc.)

文献类型： 外文期刊

第一作者： Wang, KJ

作者： Wang, KJ;Takahata, Y;Kono, Y;Kaizuma, N

作者机构：

关键词： Kunitz trypsin inhibitor;polymorphism;gene sequence;protein evolution;Glycine soja;soybean

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2005 年 112 卷 1 期

页码：

收录情况： SCI

摘要： Soybean Kunit trypsin inhibitor (SKTI) has several polymorphic types. Of these SKTI, there are large differences of nine amino acid substitutions between Tia and Tib. So far no transitional type between them has been found. A novel transitional intermediate variant between Tia and T -> ib was detected in 11 lines from 720 Japanese wild soybeans (Glycine soja Sieb. & Zucc.). This variant showed identical electrophoretic mobility to Tib in the Davis system polyacrylamide gel electrophoresis (PAGE), but higher electric points than other SKTI proteins (Tia, Tib, Tic) in isoelectric focusing PAGE. The genetic analysis of SKTI in F-2 seeds from a cross between the novel variant type and Tib showed that this variant type is inherited as codominant alleles in a multiple allelic system at an SKTI locus. This variant also showed inhibitory activity to trypsin. We propose the genetic symbol Tib(i5) for this novel variant. The sequence analysis of Tib(i5) revealed that six nucleotides were different between Tib(i5) and Tia, and the nucleotides of these mutated positions were identical to Tib. This causes substitution of five amino acids at the residue position 62 (Tyr -> Phe), 74 (Ser Arg), 114 (Met -> Val), 120 (Leu -> Ile) and 137 (Pro -> Thr). These substitutive amino acids are completely in accord with the amino acids of Tib, showing that Tib(i5) is an intermediate between Tia and Tib types. Tib(i5) type is widely distributed throughout seven separate areas from northeast to southwest Japan with a 1.5% frequency of total materials examined. This indicated that Tib(i5) type did not originate from a recent mutation event, but had spread in wild soybean from ancient times.

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