Molecular cloning, developmental expression, and tissue distribution of the gene encoding DH, PBAN and other FXPRL neuropeptides in Samia cynthia ricini

文献类型: 外文期刊

第一作者: Wei, ZJ

作者: Wei, ZJ;Zhang, TY;Sun, JS;Xu, AY;Xu, WH;Denlinger, DL

作者机构:

关键词: pheromone biosynthesis activating neuropeptide;diapause hormone;cDNA cloning;developmental expression;Samia cynthia ricini

期刊名称:JOURNAL OF INSECT PHYSIOLOGY ( 影响因子:2.354; 五年影响因子:3.045 )

ISSN: 0022-1910

年卷期: 2004 年 50 卷 12 期

页码:

收录情况: SCI

摘要: We obtained a full-length cDNA encoding diapause hormone (DH) and pheromone biosynthesis activating neuropeptide (PBAN) in Samia cynthia ricini based on both reverse transciptase-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE) strategies. The open reading frame (ORF) of this cDNA encodes a 198-amino acid precursor protein that contains a 33-aa PBAN, a 24-aa DH-like peptide, and three other neuropeptides, all of which share a common C-terminal pentapeptide motif FXPR/KL (X = G, T, S). Samia DH-like and PBAN show high homology to their counterpart in other Lepidoptera. Northern blots demonstrate the presence of a 0.8-kb transcript in the suboesophageal ganglion (SG). The DH-PBAN mRNA was detectable at much lower levels in other neural tissues, such as brain and thoracic ganglia (TG), but not in non-neural tissue, such as the midgut, silk gland, fat body or epidermis. The DH-PBAN mRNA content in the SG was measured using the combined method of quantitative RT-PCR and Southern blotting and was shown to vary with developmental stage. Using an antiserum against Helicoverpa armigera PBAN, PBAN-like immunoreactivity was detected in the SG, TG and terminal abdomen ganglion of S. cynthia ricini by whole-mount immunocytochemistry. The changes of PBAN-like immunoreactivity in the hemolymph are consistent with PBAN transcripts in the SG during pupal development. PBAN increases quickly at adult eclosion, an observation that is consistent with PBAN's key role in pheromone biosynthesis, and synthetic PBAN or brain-SG extracts successfully stimulates pheromone biosynthesis in decapitated moths. (C) 2004 Elsevier Ltd. All rights reserved.

分类号:

  • 相关文献

[1]Protein-DNA interactions in the promoter region of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide of the cotton bollworm, Helicoverpa armigera. Hong, B,Zhang, ZF,Tang, SM,Yi, YZ,Zhang, TY,Xu, WH.

[2]Identification of a POU factor involved in regulating the neuron-specific expression of the gene encoding diapause hormone and pheromone biosynthesis-activating neuropeptide in Bombyx mori. Zhang, TY,Kang, L,Zhang, ZF,Xu, WH.

[3]Localization, expression, and secretion pathway of diapause hormone in embryo and larva of the silkworm, Bombyx mori. Sun, JS,Chen, FS,Xu, WH.

[4]Molecular characterization and expression of vitellogenin gene from Spodoptera exigua exposed to cadmium stress. Zhao, Jing,Sun, Yang,Xiao, Liubin,Tan, Yongan,Bai, Lixin.

[5]Characterization of the male-specific lethal 3 gene in the oriental river prawn, Macrobrachium nipponense. Zhang, Y. P.,Sun, S. M.,Fu, H. T.,Ge, X. P.,Qiao, H.,Zhang, W. Y.,Xiong, Y. W.,Jiang, S. F.,Gong, Y. S.,Jin, S. B.. 2015

[6]CDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi. Xia, Dingguo,Xiang, Zhonghuai,Lu, Cheng,Xia, Dingguo,Wei, Yadong,Zhang, Guozheng,Zhao, Qiaoling,Zhang, Yeshun.

[7]Characterization of -catenin 1 during the gonad development in the common carp (Cyprinus carpio). Wang, Lan-mei,Zhu, Wen-bin,Fu, Jian-jun,Dong, Zai-jie,Song, Fei-biao,Dong, Juan-juan,Dong, Zai-jie.

[8]Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat. Li, AL,Kong, XY,Zhou, RH,Ma, ZY,Jia, JZ.

[9]Molecular cloning, characterization and mRNA expression of two antibacterial peptides: Crustin and anti-lipopolysaccharide factor in swimming crab Portunus trituberculatus. Yue, Feng,Pan, Luqing,Miao, Jingjing,Zhang, Lin,Li, Jian.

[10]Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses. Ji, Shaozhong,Tang, Qing,Cui, Xiaoying,Yan, Hongyang,Kan, Biao,Gao, Shouyi,Jiang, Shengjun.

[11]Cloning and characterization of ubiquitin ribosome fusion gene RpS27a, a deltamethrin-resistance-associated gene from diamondback moth (Plutella xylostella L.). Zhang, Hong,Cheng, Chen,Gu, Shuyu,Zhou, Zhou,Cheng, Luogen,Li, Fengliang.

[12]Calreticulin is required for responding to stress, foraging, and fertility in the white-tip nematode, Aphelenchoides besseyi. Feng, Hui,Wei, Lihui,Chen, Huaigu,Zhou, Yijun.

[13]The two channel catfish intelectin genes exhibit highly differential patterns of tissue expression and regulation after infection with Edwardsiella ictaluri. Takano, Tomokazu,Sha, Zhenxia,Peatman, Eric,Terhune, Jeffery,Li, Hong,Kucuktas, Huseyin,Li, Ping,Liu, Zhanjiang,Sha, Zhenxia,Edholm, Eva-Stina,Wilson, Melanie.

[14]Polygenic expression of somatostatin in orange-spotted grouper (Epinephelus coioides): Molecular cloning and distribution of the mRNAs encoding three somatostatin precursors. Ying, Y,Li, WS,Lin, HR. 2005

[15]Molecular cloning of a full-length cDNA for ECBP21 from Angelica dahurica. Mao, GH,Tang, WQ,Guo, Y,Ding, CB,Zhou, RG,Sun, DY. 2002

[16]Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution. Gui, Zhong Zheng,Kim, Bo Yeon,Lee, Kwang Sik,Wei, Ya Dong,Sohn, Hung Dae,Jin, Byung Rae,Gui, Zhong Zheng,Wei, Ya Dong,Guo, Xijie. 2008

[17]cDNA structure, genomic organization and expression patterns of visfatin in silver Prussian carp (Carassius auratus gibelio). Dong Xu,Zou Shiping,Wu Xiaoxiong,Dai Hanchuan,Zeng Cuiping,Wang Xu,Long Liangqi,Zou Shiping. 2011

[18]Cloning and characterization of squalene synthase (SQS) gene from Ganoderma lucidum. Zhao, Ming-Wen,Liang, Wan-Qi,Zhang, Da-Bing,Wang, Nan,Wang, Chen-Guang,Pan, Ying-Jie. 2007

[19]cDNA cloning of a Rab5 homologue in Babesia gibsoni. Zhou, JL,Ohashi, K,Yamasaki, M,Onuma, M. 2003

[20]Real-time Fluorescence Quantitative PCR and Prediction of Bovine Trf 2 Protein Funtion. Huai, Ya-Hong,Xu, Shang-Zhong. 2016

作者其他论文 更多>>