Fluorescent transgenic silkworm

文献类型： 外文期刊

第一作者： Zhang, F

作者： Zhang, F;Zhao, Y;Chen, X;Xu, AY;Huang, JT;Lu, CD

作者机构：

关键词： GFP;fibroin heavy chain gene;knockout

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 1999 年 31 卷 2 期

页码：

收录情况： SCI

摘要： The replacement of fibroin heavy chain gene in silkworm by site directed homologous recombination was studied. The DNA fragment, consisting of an IE promoter driving green fluorescent protein (GFP) gene as reporter flanked by pieces of the 5' and 3' sequences of the fibroin heavy chain gene of silkworm at two sides, was transferred into silkworm eggs by electroporation. Green fluorescent flecks were seen on three silkworms fifth instar among five thousand silkworms under UV light. PCR analysis proved that the GFP gene was integrated into the genome of silkworm. Southern hybridization of genomic DNA of one transgenic silkworm showed that the fibroin heavy chain gene was successfully knocked out and replaced by the reporter gene. The transgenic silkworms could grow up to the fifth instar as normal, but they could not spin silk while control ones do.

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