HPLC analyses of flavanols and phenolic acids in the fresh young shoots of tea (Camellia sinensis) grown in Australia

文献类型： 外文期刊

第一作者： Yao, LH

作者： Yao, LH;Jiang, YM;Datta, N;Singanusong, R;Liu, X;Duan, J;Raymont, K;Lisle, A;Xu, Y

作者机构：

关键词： alkaloids;flavanols;fresh shoots;HPLC;photodiode array detection;phenolic acids;tea

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2004 年 84 卷 2 期

页码：

收录情况： SCI

摘要： As part of a 4-year project to study phenolic compounds in tea shoots over the growing seasons and during black tea processing in Australia, an HPLC method was developed and optimised for the identification and quantification of phenolic compounds, mainly flavanols and phenolic acids, in fresh tea shoots. Methanol proved to be the most suitable solvent for extracting the phenolic compounds, compared with chloroform, ethyl acetate and water. Immediate analysis, by HPLC, of the methanol extract showed higher separation efficiency than analyses after being dried and redissolved. This method exhibited good repeatability (CV 3-9%) and recovery rate (88-116%). Epigallocatechin gallate alone constituted up to 115 mg/g, on a dry basis, in the single sample of Australian fresh tea shoots examined. Four catechins (catechin, gallocatechin, epicatechin and epigallocatechin) and six catechin gallates (epigallocatechin gallate, catechin gallate, epicatechin gallate, gallocatechin gallate, epicatechin digallate and epigallocatechin digallate) have been identified and quantified by this HPLC method. In addition, two major tea alkaloids, caffeine and theobromine, have been quantified, while five flavonol glycosides and six phenolic acids, including quinic acids and esters, were identified and quantified. (C) 2003 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Isocratic elution system for the determination of catechins, caffeine and gallic acid in green tea using HPLC. Wang, HF,Helliwell, K,You, XQ. 2000

[2]Oxidation of (-)-epicatechin is a precursor of litchi pericarp enzymatic browning. Liu, Liang,Xu, Yujuan,Zhang, Mingwei,Xiao, Gengsheng,Cao, Shaoqian,Deng, Qianchun.

[3]Bioassay-guided isolation and identification of active compounds from Macleaya microcarpa (Maxim) Fedde against fish pathogenic bacteria. Kang, Yu-Jun,Yi, Yang-lei,Zhang, Chao,Wang, Gao-Xue,Wu, Shu-Qin,Shi, Cun-Bin. 2013

[4]Phytochemistry and Pharmacological Activities of the Genus Gentiana (Gentianaceae). Pan, Yu,Zhao, Yan-Li,Zhang, Ji,Li, Wan-Yi,Wang, Yuan-Zhong,Pan, Yu.

[5]Novel Cyclic Hexapeptides from Marine-Derived Fungus, Aspergillus sclerotiorum PT06-1. Zheng, Jinkai,Wang, Yi,Liu, Peipei,Wang, Xin,Peng, Xiaoping,Zhu, Weiming,Zhu, Huajie,Hong, Kui.

[6]Detoxification of gramine by the cereal aphid Sitobion avenae. Cai, Qing-Nian,Han, Ying,Hu, Yuan,Zhao, Xin,Cao, Ya-Zhong.

[7]Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry. Zhang, Li-li,Shu, Shu-lan,Qian, Da-wei,Duan, Jin-ao,Zhang, Li-li,Shu, Shu-lan,Qian, Da-wei,Duan, Jin-ao,Bai, Yong-liang,Zhen Ou-yang,Liu, Li.

[8]Ultra-performance LC Separation and Quadrupole Time-of-flight MS Identification of Major Alkaloids in Plumula Nelumbinis. Lin, Zongtao,Yang, Ruinan,Li, Wei,Guan, Zheng,Chen, Ailiang. 2014

[9]Antifungal Efficacy of Phellodendron amurense Ethanol Extract against Trichophyton mentagrophytes in Rabbits. Xiao, C. W.,Ji, Q. A.,Wei, Q.,Liu, Y.,Bao, G. L.,Rajput, Z. I.. 2014

[10]Tetracyclic indole alkaloids with antinematode activity from Uncaria rhynchophylla. Kong, Fandong,Ma, Qingyun,Huang, Shengzhuo,Zhou, Liman,Dai, Haofu,Zhao, Youxing,Yang, Shuang,Fu, Linran,Yu, Zhifang. 2017

[11]DETERMINATION OF LOTUS LEAF ALKALOIDS BY SOLID PHASE EXTRACTION COMBINED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DIODE ARRAY AND TANDEM MASS SPECTROMETRY DETECTION. Chen, Sha,Liu, Yanling,Li, Shaohua,Chen, Sha,Zhang, Haohao,Fang, Jinbao.

[12]Two Stemona alkaloids from Stemona sessilifolia (Miq.) Miq.. Dong, Jian-Li,Yang, Zhong-Duo,Xue, Hong-Yan,Shu, Zong-Mei,Zhou, Shuang-Yan,Yao, Xiao-Jun,Xue, Hong-Yan.

[13]Simultaneous determination of flavanones, hydroxycinnamic acids and alkaloids in citrus fruits by HPLC-DAD-ESI/MS. Liu, Guozhu,Chen, Bo,Yao, Shouzhuo,He, Dongxiu,Shan, Yang,Wu, Yuehui.

[14]Microwave-assisted extraction of three bioactive alkaloids from Peganum harmala L. and their acaricidal activity against Psoroptes cuniculi in vitro. Shang, Xiaofei,Guo, Xiao,Li, Bing,Pan, Hu,Zhang, Jiyu,Miao, Xiaolou,Zhang, Yu.

[15]Anti-endotoxin and anti-inflammatory effects of Chinese herbal medicinal alkaloid ingredients in vivo. Hu, Yiyi,Mao, Aihua,Yu, Zhengyu,He, Kongwang,Hu, Yiyi,Mao, Aihua,Yu, Zhengyu,He, Kongwang.

[16]Trigonelline, a naturally occurring constituent of green coffee beans behind the mutagenic activity of roasted coffee?. Wu, X,Skog, K,Jagerstad, M.

[17]Complex enzyme hydrolysis releases antioxidative phenolics from rice bran. Liu, Lei,Wen, Wei,Zhang, Ruifen,Wei, Zhencheng,Deng, Yuanyuan,Xiao, Juan,Zhang, Mingwei. 2017

[18]Chemical constituents from Orobanche cernua Loefling. Qu, Zheng-yi,Zhang, Yu-wei,Yao, Chun-lin,Jin, Yin-ping,Zheng, Pei-he,Sun, Cheng-he,Liu, Jun-xia,Wang, Yu-shuai,Wang, Ying-ping.

[19]Phenolic acids, anthocyanins, proanthocyanidins, antioxidant activity, minerals and their correlations in non-pigmented, red, and black rice. Shao, Yafang,Hu, Zhanqiang,Yu, Yonghong,Mou, Renxiang,Zhu, Zhiwei,Beta, Trust. 2018

[20]The interaction of phenolic acids with Fe(III) in the presence of citrate as studied by isothermal titration calorimetry. Yang, Senpei,Bai, Guangling,Chen, Lingli,Shen, Qun,Zhao, Guanghua,Diao, Xianmin. 2014

作者其他论文 更多>>

微信小程序