Cloning, expressing and functional analysis of SjMF4, a novel Schistosoma japonicum gene
文献类型: 外文期刊
第一作者: Zhang, L
作者: Zhang, L;Cheng, GF;Fu, ZQ;Jin, YM;Feng, XG;Yuan, CX;Cai, YM;Lin, JJ
作者机构:
关键词: Microtus fortis;Schistosoma japonicum;SjMF4;protection
期刊名称:ACTA BIOCHIMICA ET BIOPHYSICA SINICA ( 影响因子:3.848; 五年影响因子:3.67 )
ISSN: 0582-9879
年卷期: 2003 年 35 卷 12 期
页码:
收录情况: SCI
摘要: Based on the phenomenon of the natural anti-schistosomiasis in Microtus fortis, the sera from normal Microtus fortis were employed to immunoscreen the cDNA library of adult Schistosoma japonicum (Chinese strain), two positive clones were obtained, RACE technique was further applied to amplify one of the clones, and a cDNA fragment with an ORF was identified. Sequencing revealed that it was a novel gene of Schistosoma japonicum, and it was named SjMF4 (Schistoma japonicum Microtus fortis 4). Then the structure and functional motifs of SjMF4 were analysed. The gene was subcloned into pET-28a (+) vector; the recombinant protein showed good antigenicity in Western blotting. The gene was further subcloned into eukaryotic expression vector pcDNA3 to construct the DNA vaccine containing SjMF4. Immune experiments in mice showed significant protection that the recombinant plasmid did induce 28.64% +/- 3.82% worm reduction and 21.73% +/- 3.98% egg reduction than controls against the Schistosoma japonicum cercaria challenge.
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