Cloning and expression of actin gene of Schistosoma japonicum Chinese strain

文献类型： 外文期刊

第一作者： Zhu, JG

作者： Zhu, JG;Lin, JJ;Feng, XG;Wu, XF;Zhou, YC;Cai, YM

作者机构：

关键词： Schistosoma japonicum;actin;gene cloning;gene expression

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 2000 年 32 卷 5 期

页码：

收录情况： SCI

摘要： A 1 131 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma japonicum (Chinese strain) mRNA with primers designed according to published SmAct2 encoding Schistosoma mansoni actin. Sequence analysis indicated that this fragment, with 92 % homology to SmAct2, was a complete open reading fragment (ORF) of actin gene of Schistosoma japonicum (Chinese strain). This gene was cloned into the expression vector pET28a(+) and subsequently expressed in Escerichia coli. SDS-PAGE revealed that the molecular weight of this expressed product was 47 kD. Western blotting showed that the recombinant protein had good reactivity with the rabbit serum immunized with Sj worm antigen, indicating that this gene encode actin of Schistosoma japonicum (Chinese strain).

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