Identification of cry1I-type genes from Bacillus thuringiensis strains and characterization of a novel cry1I-type gene

文献类型： 外文期刊

第一作者： Zhang, J

作者： Zhang, J;Gu, AX;Wu, Y;Han, LL;He, KL;Chen, ZY;Yao, J;Hu, YQ;Li, GX;Huang, DF

作者机构：

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

ISSN： 0099-2240

年卷期： 2003 年 69 卷 9 期

页码：

收录情况： SCI

摘要： A PCR-restriction fragment length polymorphism method for identification of cry1I-type genes from Bacillus thuringiensis was established by designing a pair of universal primers based on the conserved regions of the genes to amplify 1,548-bp crylI-type gene fragments. Amplification products were digested with the Bsp119I and BanI enzymes, and four kinds of known crylI-type genes were successfully identified. The results showed that cry1I-type genes appeared in 95 of 115 B. thuringiensis isolates and 7 of 13 standard strains. A novel cty1I-type gene was found in one standard strain and six isolates. The novel cry1I gene was cloned from B. thuringiensis isolate Btc007 and subcloned into vector pET-21b. Then it was overexpressed in Escherichia coli BL21(DE3). The expressed product was shown to be toxic to the diamondback moth (Plutella xylostella), Asian corn borer (Ostrinia furnacalis), and soybean pod borer (Leguminivora glycinivorella). However, it was not toxic to the cotton bollworm (Helicoverpa armigera), beet armyworm (Spodoptera exigua), or elm leaf beetle (Pyrrhalta aenescens) in bioassays. Subsequently, the Cry protein encoded by this novel cry gene was designated Cry1Ie1 by the B. thuringiensis delta-endotoxin nomenclature committee.

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